EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.8 | E196A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
2.4.2.8 | E196D | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
2.4.2.8 | E196Q | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
2.4.2.8 | E196R | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
2.4.2.8 | E196V | a naturally occuring mutation that leads to the Lesch-Nyhan syndrome | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
2.4.2.8 | 0.0024 | - |
IMP | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.0028 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
2.4.2.8 | 0.0036 | - |
hypoxanthine | mutant E196Q | Homo sapiens | |
2.4.2.8 | 0.0038 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.0049 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.0063 | - |
hypoxanthine | mutant E196A | Homo sapiens | |
2.4.2.8 | 0.0147 | - |
IMP | mutant E196Q | Homo sapiens | |
2.4.2.8 | 0.0175 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.0191 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.0726 | - |
IMP | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.185 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
2.4.2.8 | 0.659 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
2.4.2.8 | 0.968 | - |
diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 1.043 | - |
IMP | mutant E196A | Homo sapiens | |
2.4.2.8 | 1.156 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.8 | Homo sapiens | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.2.8 | IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
r | |
2.4.2.8 | additional information | substrate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.8 | HPRT | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.038 | - |
IMP | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.041 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.18 | - |
IMP | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.23 | - |
IMP | mutant E196A | Homo sapiens | |
2.4.2.8 | 0.33 | - |
diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.51 | - |
IMP | mutant E196Q | Homo sapiens | |
2.4.2.8 | 2.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 2.6 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 2.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
2.4.2.8 | 3.8 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
2.4.2.8 | 10.9 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
2.4.2.8 | 13.7 | - |
hypoxanthine | mutant E196Q | Homo sapiens | |
2.4.2.8 | 13.8 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
2.4.2.8 | 14.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 20.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens | |
2.4.2.8 | 20.3 | - |
hypoxanthine | mutant E196A | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.0003 | - |
diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.002 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.0024 | - |
IMP | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.004 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
2.4.2.8 | 0.016 | - |
IMP | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.02 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens | |
2.4.2.8 | 0.034 | - |
IMP | mutant E196Q | Homo sapiens | |
2.4.2.8 | 0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
2.4.2.8 | 0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
2.4.2.8 | 0.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 0.7 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
2.4.2.8 | 1 | - |
IMP | mutant E196A | Homo sapiens | |
2.4.2.8 | 1.4 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
2.4.2.8 | 2.8 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
2.4.2.8 | 3.2 | - |
hypoxanthine | mutant E196A | Homo sapiens | |
2.4.2.8 | 3.8 | - |
hypoxanthine | mutant E196Q | Homo sapiens |