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Literature summary extracted from

  • Canyuk, B.; E-Wan, A.; Keawwijit, W.; Nualnoi, T.; Sirisatean, L.; Tansakul, P.; Tanthana, C.
    The role for glutamic acid at position 196 in human hypoxanthine phosphoribosyltransferase (HPRT) as investigated using site-directed mutagenesis (2008), Nucleosides Nucleotides Nucleic Acids, 27, 894-899.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 E196A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.2.8 E196D site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.2.8 E196Q site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.2.8 E196R site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.2.8 E196V a naturally occuring mutation that leads to the Lesch-Nyhan syndrome Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 additional information
-
additional information steady-state kinetics Homo sapiens
2.4.2.8 0.0024
-
IMP wild-type enzyme Homo sapiens
2.4.2.8 0.0028
-
hypoxanthine mutant E196R Homo sapiens
2.4.2.8 0.0036
-
hypoxanthine mutant E196Q Homo sapiens
2.4.2.8 0.0038
-
hypoxanthine wild-type enzyme Homo sapiens
2.4.2.8 0.0049
-
hypoxanthine mutant E196D Homo sapiens
2.4.2.8 0.0063
-
hypoxanthine mutant E196A Homo sapiens
2.4.2.8 0.0147
-
IMP mutant E196Q Homo sapiens
2.4.2.8 0.0175
-
diphosphate wild-type enzyme Homo sapiens
2.4.2.8 0.0191
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
2.4.2.8 0.0726
-
IMP mutant E196D Homo sapiens
2.4.2.8 0.185
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
2.4.2.8 0.2
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
2.4.2.8 0.659
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
2.4.2.8 0.968
-
diphosphate mutant E196D Homo sapiens
2.4.2.8 1.043
-
IMP mutant E196A Homo sapiens
2.4.2.8 1.156
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
IMP + diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Homo sapiens IMP + diphosphate
-
r
2.4.2.8 additional information substrate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HPRT
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 0.038
-
IMP wild-type enzyme Homo sapiens
2.4.2.8 0.041
-
diphosphate wild-type enzyme Homo sapiens
2.4.2.8 0.18
-
IMP mutant E196D Homo sapiens
2.4.2.8 0.23
-
IMP mutant E196A Homo sapiens
2.4.2.8 0.33
-
diphosphate mutant E196D Homo sapiens
2.4.2.8 0.51
-
IMP mutant E196Q Homo sapiens
2.4.2.8 2.5
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
2.4.2.8 2.6
-
hypoxanthine wild-type enzyme Homo sapiens
2.4.2.8 2.8
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
2.4.2.8 3.8
-
hypoxanthine mutant E196R Homo sapiens
2.4.2.8 10.9
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
2.4.2.8 13.7
-
hypoxanthine mutant E196Q Homo sapiens
2.4.2.8 13.8
-
hypoxanthine mutant E196D Homo sapiens
2.4.2.8 14.2
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
2.4.2.8 20.3
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens
2.4.2.8 20.3
-
hypoxanthine mutant E196A Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.8 0.0003
-
diphosphate mutant E196D Homo sapiens
2.4.2.8 0.002
-
diphosphate wild-type enzyme Homo sapiens
2.4.2.8 0.0024
-
IMP mutant E196D Homo sapiens
2.4.2.8 0.004
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
2.4.2.8 0.016
-
IMP wild-type enzyme Homo sapiens
2.4.2.8 0.02
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens
2.4.2.8 0.034
-
IMP mutant E196Q Homo sapiens
2.4.2.8 0.05
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
2.4.2.8 0.08
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
2.4.2.8 0.1
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
2.4.2.8 0.7
-
hypoxanthine wild-type enzyme Homo sapiens
2.4.2.8 1
-
IMP mutant E196A Homo sapiens
2.4.2.8 1.4
-
hypoxanthine mutant E196R Homo sapiens
2.4.2.8 2.8
-
hypoxanthine mutant E196D Homo sapiens
2.4.2.8 3.2
-
hypoxanthine mutant E196A Homo sapiens
2.4.2.8 3.8
-
hypoxanthine mutant E196Q Homo sapiens