Literature summary extracted from

Kaminski, P.A.; Dugue, L.; Pochet, S.
Expanding substrate specificity of nucleoside 2-deoxyribosyltransferase (2008), Nucleic Acids Symp. Ser., 52, 495-496.

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.2.6	A15C	is not capable of improving 2',3'-dideoxyribose transfer reaction	Limosilactobacillus fermentum
2.4.2.6	A15S	Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical	Limosilactobacillus fermentum
2.4.2.6	A15T	a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type)	Limosilactobacillus fermentum
2.4.2.6	G9S	a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity	Lactobacillus leichmannii

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.6	Lactobacillus leichmannii	-	-	-
2.4.2.6	Limosilactobacillus fermentum	Q6YNI5	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.2.6	0.01	-	wild-type, 2',3'-dideoxyadenosine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.01	-	wild-type, 2',3'-dideoxycytidine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.03	-	wild-type, 2',3'-dideoxyadenosine and cytosine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.03	-	wild-type, 2,3'-dideoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.036	-	wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.05	-	wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.06	-	wild-type, 2',3'-dideoxycytidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.42	-	mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.46	-	mutant A15T, 2'-deoxyadenosine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	0.5	-	mutant A15T, 2,3'-dideoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	1.1	-	mutant A15T, 2'-deoxyadenosine and cytosine as substrates	Limosilactobacillus fermentum
2.4.2.6	1.29	-	mutant A15T, 2'-deoxycytidine and hypoxanthine as substrates	Limosilactobacillus fermentum
2.4.2.6	1.35	-	mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates	Limosilactobacillus fermentum
2.4.2.6	1.47	-	mutant A15T, 2',3'-dideoxycytidine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	1.9	-	mutant A15T, 2'-deoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	6.8	-	mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates	Limosilactobacillus fermentum
2.4.2.6	6.9	-	wild-type, 2'-deoxycytidine and hypoxanthine as substrates	Limosilactobacillus fermentum
2.4.2.6	7.1	-	mutant A15T, 2',3'-dideoxycytidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	7.11	-	mutant A15T, 2'-deoxycytidine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	8.4	-	wild-type, 2'-deoxyadenosine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	9.28	-	mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	9.3	-	mutant A15T, 2'-deoxycytidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	20.1	-	wild-type, 2'-deoxythymidine and adenine as substrates	Limosilactobacillus fermentum
2.4.2.6	32	-	wild-type, 2'-deoxycytidine and thymine as substrates	Limosilactobacillus fermentum
2.4.2.6	33.2	-	wild-type, 2'-deoxyadenosine and cytosine as substrates	Limosilactobacillus fermentum
2.4.2.6	83.7	-	wild-type, 2'-deoxycytidine and adenine as substrates	Limosilactobacillus fermentum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.4.2.6	2',3'-dideoxyadenosine + cytosine	-	Limosilactobacillus fermentum	2',3'-dideoxycytidine + adenine	-	?
2.4.2.6	2',3'-dideoxyadenosine + thymine	-	Limosilactobacillus fermentum	2',3'-dideoxythymidine + adenine	-	?
2.4.2.6	2',3'-dideoxycytidine + adenine	-	Limosilactobacillus fermentum	2',3'-dideoxyadenosine + cytosine	-	?
2.4.2.6	2',3'-dideoxycytidine + hypoxanthine	-	Limosilactobacillus fermentum	2',3'-dideoxyinosine + cytosine	-	?
2.4.2.6	2',3'-dideoxycytidine + thymine	-	Limosilactobacillus fermentum	2',3'-dideoxythymidine + cytosine	-	?
2.4.2.6	2',3'-dideoxythymidine + adenine	-	Limosilactobacillus fermentum	2',3'-dideoxyadenosine + thymine	-	?
2.4.2.6	2',3'didehydro-2',3'-dideoxythymidine + adenine	-	Limosilactobacillus fermentum	2',3'didehydro-2',3'-dideoxyadenosine + thymine	-	?
2.4.2.6	2'-deoxyadenosine + cytosine	-	Limosilactobacillus fermentum	2'-deoxycytidine + adenine	-	?
2.4.2.6	2'-deoxyadenosine + thymine	-	Limosilactobacillus fermentum	2'-deoxythymidine + adenine	-	?
2.4.2.6	2'-deoxycytidine + adenine	-	Limosilactobacillus fermentum	2'-deoxyadenosine + cytosine	-	?
2.4.2.6	2'-deoxycytidine + thymine	-	Limosilactobacillus fermentum	2'-deoxythymidine + cytosine	-	?
2.4.2.6	2'-deoxythymidine + adenine	-	Limosilactobacillus fermentum	2'-deoxyadenosine + thymine	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.2.6	NTD	-	Lactobacillus leichmannii
2.4.2.6	NTD	-	Limosilactobacillus fermentum
2.4.2.6	nucleoside 2'-deoxyribosyltransferase	-	Lactobacillus leichmannii
2.4.2.6	nucleoside 2'-deoxyribosyltransferase	-	Limosilactobacillus fermentum