BRENDA - Enzyme Database show

Expanding substrate specificity of nucleoside 2-deoxyribosyltransferase

Kaminski, P.A.; Dugue, L.; Pochet, S.; Nucleic Acids Symp. Ser. 52, 495-496 (2008)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
A15C
is not capable of improving 2',3'-dideoxyribose transfer reaction
Lactobacillus fermentum
2.4.2.6
A15S
Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical
Lactobacillus fermentum
2.4.2.6
A15T
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type)
Lactobacillus fermentum
2.4.2.6
G9S
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity
Lactobacillus leichmannii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus fermentum
Q6YNI5
-
-
2.4.2.6
Lactobacillus leichmannii
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.01
-
wild-type, 2',3'-dideoxyadenosine and thymine as substrates; wild-type, 2',3'-dideoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.03
-
wild-type, 2',3'-dideoxyadenosine and cytosine as substrates; wild-type, 2,3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.036
-
wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.05
-
wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.06
-
wild-type, 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.42
-
mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.46
-
mutant A15T, 2'-deoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.5
-
mutant A15T, 2,3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.1
-
mutant A15T, 2'-deoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.29
-
mutant A15T, 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
1.35
-
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.47
-
mutant A15T, 2',3'-dideoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
1.9
-
mutant A15T, 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
6.8
-
mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
6.9
-
wild-type, 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
7.1
-
mutant A15T, 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
7.11
-
mutant A15T, 2'-deoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
8.4
-
wild-type, 2'-deoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
9.28
-
mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
9.3
-
mutant A15T, 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
20.1
-
wild-type, 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
32
-
wild-type, 2'-deoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
33.2
-
wild-type, 2'-deoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
83.7
-
wild-type, 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2',3'-dideoxyadenosine + cytosine
-
705990
Lactobacillus fermentum
2',3'-dideoxycytidine + adenine
-
-
-
?
2.4.2.6
2',3'-dideoxyadenosine + thymine
-
705990
Lactobacillus fermentum
2',3'-dideoxythymidine + adenine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
705990
Lactobacillus fermentum
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
705990
Lactobacillus fermentum
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + thymine
-
705990
Lactobacillus fermentum
2',3'-dideoxythymidine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
705990
Lactobacillus fermentum
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
705990
Lactobacillus fermentum
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxyadenosine + cytosine
-
705990
Lactobacillus fermentum
2'-deoxycytidine + adenine
-
-
-
?
2.4.2.6
2'-deoxyadenosine + thymine
-
705990
Lactobacillus fermentum
2'-deoxythymidine + adenine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
705990
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + thymine
-
705990
Lactobacillus fermentum
2'-deoxythymidine + cytosine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
705990
Lactobacillus fermentum
2'-deoxyadenosine + thymine
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
A15C
is not capable of improving 2',3'-dideoxyribose transfer reaction
Lactobacillus fermentum
2.4.2.6
A15S
Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical
Lactobacillus fermentum
2.4.2.6
A15T
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type)
Lactobacillus fermentum
2.4.2.6
G9S
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity
Lactobacillus leichmannii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.01
-
wild-type, 2',3'-dideoxyadenosine and thymine as substrates; wild-type, 2',3'-dideoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.03
-
wild-type, 2',3'-dideoxyadenosine and cytosine as substrates; wild-type, 2,3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.036
-
wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.05
-
wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.06
-
wild-type, 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.42
-
mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.46
-
mutant A15T, 2'-deoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.5
-
mutant A15T, 2,3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.1
-
mutant A15T, 2'-deoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.29
-
mutant A15T, 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
1.35
-
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.47
-
mutant A15T, 2',3'-dideoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
1.9
-
mutant A15T, 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
6.8
-
mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
6.9
-
wild-type, 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
7.1
-
mutant A15T, 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
7.11
-
mutant A15T, 2'-deoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
8.4
-
wild-type, 2'-deoxyadenosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
9.28
-
mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
9.3
-
mutant A15T, 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
20.1
-
wild-type, 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
32
-
wild-type, 2'-deoxycytidine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
33.2
-
wild-type, 2'-deoxyadenosine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
83.7
-
wild-type, 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2',3'-dideoxyadenosine + cytosine
-
705990
Lactobacillus fermentum
2',3'-dideoxycytidine + adenine
-
-
-
?
2.4.2.6
2',3'-dideoxyadenosine + thymine
-
705990
Lactobacillus fermentum
2',3'-dideoxythymidine + adenine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
705990
Lactobacillus fermentum
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
705990
Lactobacillus fermentum
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + thymine
-
705990
Lactobacillus fermentum
2',3'-dideoxythymidine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
705990
Lactobacillus fermentum
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
705990
Lactobacillus fermentum
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxyadenosine + cytosine
-
705990
Lactobacillus fermentum
2'-deoxycytidine + adenine
-
-
-
?
2.4.2.6
2'-deoxyadenosine + thymine
-
705990
Lactobacillus fermentum
2'-deoxythymidine + adenine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
705990
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + thymine
-
705990
Lactobacillus fermentum
2'-deoxythymidine + cytosine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
705990
Lactobacillus fermentum
2'-deoxyadenosine + thymine
-
-
-
?