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Literature summary extracted from

  • Kimura, S.; Suzuki, T.
    Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA (2010), Nucleic Acids Res., 38, 1341-1352.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.198
-
 Escherichia coli
2.1.1.199
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.198 S-adenosyl-L-methionine + cytidine1402 in 16S rRNA Escherichia coli the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
 ?
2.1.1.199 S-adenosyl-L-methionine + cytosine1402 in 16S rRNA Escherichia coli the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.198 Escherichia coli P67087
-
-
2.1.1.199 Escherichia coli P60390
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.198 recombinant protein with N-terminal 6His-tags Escherichia coli
2.1.1.199 recombinant protein with N-terminal 6His-tags Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.198 S-adenosyl-L-methionine + cytidine1402 in 16S rRNA the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
 ?
2.1.1.199 S-adenosyl-L-methionine + cytosine1402 in 16S rRNA the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome Escherichia coli S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.198 RsmI
-
 Escherichia coli
2.1.1.198 YraL
-
 Escherichia coli
2.1.1.199 mraW
-
 Escherichia coli
2.1.1.199 RsmH
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.198 malfunction strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme Escherichia coli
2.1.1.198 physiological function both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons Escherichia coli
2.1.1.199 malfunction strain DELTAmraW produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme Escherichia coli
2.1.1.199 physiological function both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons Escherichia coli