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Literature summary extracted from

  • Ho, M.C.; Menetret, J.F.; Tsuruta, H.; Allen, K.N.
    The origin of the electrostatic perturbation in acetoacetate decarboxylase (2009), Nature, 459, 393-397.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.4 expressed in Escherichia coli BL21(DE3) cells Clostridium acetobutylicum
4.1.1.4 expressed in Escherichia coli BL21(DE3) cells Chromobacterium violaceum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.4 AAD complexed with 2,4-pentanedione, hanging drop vapor diffusion method, using 18-20% (v/v) glycerol, 40 mM phosphate buffer, pH 5.95, 100 mM sarcosine and 14-15% (w/v) PEG 3350 Clostridium acetobutylicum
4.1.1.4 AAD complexed with 2,4-pentanedione, hanging drop vapor diffusion method, using 18-20% (v/v) glycerol, 40 mM phosphate buffer, pH 5.95, 100 mM sarcosine and 14-15% (w/v) PEG 3350 Chromobacterium violaceum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.4 E61Q the catalytic activity of the mutant shows a decrease in kcat (about 20fold with no change in Km) Clostridium acetobutylicum
4.1.1.4 E61Q the catalytic activity of the mutant shows a decrease in kcat (about 20fold with no change in Km) Chromobacterium violaceum
4.1.1.4 E76Q the catalytic activity of the mutant shows a decrease in kcat, the mutant exhibits a slight downward shift of the pH optimum Clostridium acetobutylicum
4.1.1.4 E76Q the catalytic activity of the mutant shows a decrease in kcat, the mutant exhibits aslight downward shift of the pH optimum Chromobacterium violaceum
4.1.1.4 R29Q the catalytic activity of Arg29Gln does not increase at pH values above the wild type optimum for AAD of about 5.4 Clostridium acetobutylicum
4.1.1.4 R29Q the catalytic activity of Arg29Gln does not increase at pH values above the wild type optimum for AAD of about 5.4 Chromobacterium violaceum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.4 2,4-Pentanedione potent inhibitor Chromobacterium violaceum
4.1.1.4 2,4-Pentanedione potent inhibitor Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.4 4.1
-
 acetoacetate at 25°C, in 50 mM phosphate buffer, pH 5.95 Clostridium acetobutylicum
4.1.1.4 5.7
-
 acetoacetate at 25°C, in 50 mM phosphate buffer, pH 5.95 Chromobacterium violaceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.4 365000
-
-
 Clostridium acetobutylicum
4.1.1.4 365000
-
-
 Chromobacterium violaceum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.4 Chromobacterium violaceum Q7NSA6
-
-
4.1.1.4 Clostridium acetobutylicum P23670
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.4 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and S-200 gel filtration Clostridium acetobutylicum
4.1.1.4 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and S-200 gel filtration Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.4 2,4-pentanedione
-
 Chromobacterium violaceum ?
-
 ?
4.1.1.4 acetoacetate + H+
-
 Clostridium acetobutylicum acetone + CO2
-
 ?
4.1.1.4 acetoacetate + H+
-
 Chromobacterium violaceum acetone + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.4 homodecamer x-ray crystallography Clostridium acetobutylicum
4.1.1.4 homodecamer x-ray crystallography Chromobacterium violaceum

Synonyms

EC Number Synonyms Comment Organism
4.1.1.4 AAD
-
 Clostridium acetobutylicum
4.1.1.4 AAD
-
 Chromobacterium violaceum
4.1.1.4 AADase
-
 Clostridium acetobutylicum
4.1.1.4 AADase
-
 Chromobacterium violaceum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.4 165
-
 acetoacetate at 25°C, in 50 mM phosphate buffer, pH 5.95 Clostridium acetobutylicum
4.1.1.4 349
-
 acetoacetate at 25°C, in 50 mM phosphate buffer, pH 5.95 Chromobacterium violaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.4 5.4
-
-
 Clostridium acetobutylicum
4.1.1.4 5.4
-
-
 Chromobacterium violaceum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.4 0.0007
-
 2,4-Pentanedione at 25°C, in 50 mM phosphate buffer, pH 5.95 Clostridium acetobutylicum