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Literature summary extracted from

  • Dennis, R.J.; Taylor, E.J.; Macauley, M.S.; Stubbs, K.A.; Turkenburg, J.P.; Hart, S.J.; Black, G.N.; Vocadlo, D.J.; Davies, G.J.
    Structure and mechanism of a bacterial beta -glucosaminidase having O-GlcNAcase activity (2006), Nat. Struct. Mol. Biol., 13, 365-371.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.169 soluble BtGH84 is expressed in the cytoplasm of Escherichia coli Bacteroides thetaiotaomicron

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.169 X-ray crystal structure of BtGH84 in complex with the inhibitor NAG-thiazoline. Hanging drop vapor diffusion method Bacteroides thetaiotaomicron

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.169 D242A site-directed mutant, 2800fold lower catalytic efficiency than wild-type Bacteroides thetaiotaomicron
3.2.1.169 D242N site-directed mutant Bacteroides thetaiotaomicron
3.2.1.169 D243A site-directed mutant, 110fold lower catalytic efficiency than wild-type Bacteroides thetaiotaomicron
3.2.1.169 D243N site-directed mutant Bacteroides thetaiotaomicron
3.2.1.169 N372A site-directed mutant Bacteroides thetaiotaomicron
3.2.1.169 Y137F site-directed mutant Bacteroides thetaiotaomicron
3.2.1.169 Y282F site-directed mutant Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.169 1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline NAG-thiazoline Bacteroides thetaiotaomicron
3.2.1.169 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc Bacteroides thetaiotaomicron
3.2.1.169 streptozotocin STZ Bacteroides thetaiotaomicron

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.169 0.016
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide D243N mutant Bacteroides thetaiotaomicron
3.2.1.169 0.092
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide D243A mutant Bacteroides thetaiotaomicron
3.2.1.169 0.28
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Bacteroides thetaiotaomicron
3.2.1.169 0.3
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
 Bacteroides thetaiotaomicron
3.2.1.169 0.41
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide Y282F mutant Bacteroides thetaiotaomicron
3.2.1.169 1.18
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide Y137F mutant Bacteroides thetaiotaomicron
3.2.1.169 1.4
-
 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide
-
 Bacteroides thetaiotaomicron
3.2.1.169 1.47
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide D242N mutant Bacteroides thetaiotaomicron
3.2.1.169 4.4
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide D242A mutant Bacteroides thetaiotaomicron
3.2.1.169 36
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide N372A mutant Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.169 additional information Bacteroides thetaiotaomicron enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Bacteroides thetaiotaomicron
-
-
-
3.2.1.169 Bacteroides thetaiotaomicron Q89ZI2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.169 affinity chromatography using nickel-affinity chromatography followed by gel filtration Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 4-nitrophenyl 2-acetamido-2-deoxy-1-thio-beta-D-glucopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-nitrobenzenethiol + N-acetyl-D-glucosamine
-
 ?
3.2.1.169 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O
-
 Bacteroides thetaiotaomicron 4-nitrophenol + N-acetyl-beta-D-thioglucosamine
-
 ?
3.2.1.169 additional information enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide Bacteroides thetaiotaomicron ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.169 dimer
-
 Bacteroides thetaiotaomicron

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 thioglycosidase
-
 Bacteroides thetaiotaomicron
3.2.1.169 BtGH84
-
 Bacteroides thetaiotaomicron
3.2.1.169 O-GlcNAcase
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.169 37
-
 assay at Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 7.4
-
 assay at Bacteroides thetaiotaomicron
3.2.1.169 6
-
 bell-shaped pH-activity profile Bacteroides thetaiotaomicron