Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ting, L.M.; Gissot, M.; Coppi, A.; Sinnis, P.; Kim, K.
    Attenuated Plasmodium yoelii lacking purine nucleoside phosphorylase confer protective immunity (2008), Nat. Med., 14, 954-958.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0047
-
 Inosine pH 7.4 Plasmodium falciparum
2.4.2.1 0.005
-
 Methylthioinosine pH 7.4 Plasmodium yoelii
2.4.2.1 0.0086
-
 Inosine pH 7.4 Plasmodium yoelii
2.4.2.1 0.0089
-
 guanosine pH 7.4 Plasmodium yoelii
2.4.2.1 0.0094
-
 guanosine pH 7.4 Plasmodium falciparum
2.4.2.1 0.0108
-
 Methylthioinosine pH 7.4 Plasmodium falciparum
2.4.2.1 0.079
-
 2'-deoxyinosine pH 7.4 Plasmodium yoelii
2.4.2.1 0.091
-
 2'-deoxyinosine pH 7.4 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Plasmodium falciparum
-
-
-
2.4.2.1 Plasmodium yoelii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyinosine + phosphate
-
 Plasmodium falciparum alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Plasmodium yoelii alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
 ?
2.4.2.1 guanosine + phosphate
-
 Plasmodium falciparum guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Plasmodium yoelii guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Plasmodium yoelii hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 methylthioinosine + phosphate
-
 Plasmodium falciparum methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 methylthioinosine + phosphate
-
 Plasmodium yoelii methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 0.9
-
 2'-deoxyinosine pH 7.4 Plasmodium falciparum
2.4.2.1 1.1
-
 Inosine pH 7.4 Plasmodium falciparum
2.4.2.1 1.2
-
 2'-deoxyinosine pH 7.4 Plasmodium yoelii
2.4.2.1 2.6
-
 guanosine pH 7.4 Plasmodium falciparum
2.4.2.1 2.6
-
 Methylthioinosine pH 7.4 Plasmodium falciparum
2.4.2.1 3.4
-
 Methylthioinosine pH 7.4 Plasmodium yoelii
2.4.2.1 3.5
-
 Inosine pH 7.4 Plasmodium yoelii
2.4.2.1 3.8
-
 guanosine pH 7.4 Plasmodium yoelii

General Information

EC Number General Information Comment Organism
2.4.2.1 physiological function parasites lacking purine nucelotide phosphorylase due to gene disruption are attenuated and clear in mice. Although able to form gametocytes, purine nucelotide phosphorylase-deficient parasites do not form oocysts in mosquito midguts and are not transmitted from mosquitoes to mice. Mice given purine nucelotide phosphorylase-deficient parasites are immune to subsequent challenge to a lethal inoculum of Plasmodium yoelii YM and to challenge from strain Plasmodium yoelii 17XNL Plasmodium yoelii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 9.9
-
 2'-deoxyinosine pH 7.4 Plasmodium falciparum
2.4.2.1 15.2
-
 2'-deoxyinosine pH 7.4 Plasmodium yoelii
2.4.2.1 234
-
 Inosine pH 7.4 Plasmodium falciparum
2.4.2.1 240.7
-
 Methylthioinosine pH 7.4 Plasmodium falciparum
2.4.2.1 289
-
 guanosine pH 7.4 Plasmodium falciparum
2.4.2.1 4069
-
 Inosine pH 7.4 Plasmodium yoelii
2.4.2.1 4269
-
 guanosine pH 7.4 Plasmodium yoelii
2.4.2.1 6800
-
 Methylthioinosine pH 7.4 Plasmodium yoelii