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Literature summary extracted from

  • Horinouchi, N.; Kawano, T.; Sakai, T.; Matsumoto, S.; Sasaki, M.; Mikami, Y.; Ogawa, J.; Shimizu, S.
    Screening and characterization of a phosphopentomutase useful for enzymatic production of 2-deoxyribonucleoside (2009), N. Biotechnol., 26, 75-82.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.4.2.7 biotechnology the Escherichia coli expressing Bacillus sphaericus phosphopentomutase is an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds Lysinibacillus sphaericus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.7 for expression in Escherichia coli BL21 star DE3 cells Escherichia coli
5.4.2.7 into the vectors pT7BlueT, pBluescript II SK, pTK7, and pET-21a+ for expression in Escherichia coli BL21 star DE3 cells Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.7 Mn2+
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.7 43000
-
 determined by SDS-PAGE Lysinibacillus sphaericus
5.4.2.7 46000
-
 determined by SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.7 alpha-D-ribose 1-phosphate Lysinibacillus sphaericus
-
 D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate Escherichia coli
-
 D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate Lysinibacillus sphaericus AKU 229
-
 D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.7 Escherichia coli P0A6K6
-
-
5.4.2.7 Lysinibacillus sphaericus B3ITC5
-
-
5.4.2.7 Lysinibacillus sphaericus AKU 229 B3ITC5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Lysinibacillus sphaericus D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Escherichia coli D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Lysinibacillus sphaericus AKU 229 D-ribose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.7 phosphopentomutase
-
 Lysinibacillus sphaericus
5.4.2.7 phosphopentomutase
-
 Escherichia coli
5.4.2.7 PPMase
-
 Lysinibacillus sphaericus
5.4.2.7 PPMase
-
 Escherichia coli