EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.25 | adeno-associated vectors expressing wild-type (AAV5-wtCYP46A1) or mutated (AAV5-mtCYP46A1) CYP46A1 cDNA tagged with the hemaglutinin epitope injected in hippocampus, frontal, and parietal cortices of both hemispheres of 3-month-old APP23 mice. AAV5-wtCYP46A1 vector injection in the cortex and hippocampus of amyloid precursor protein/presenilin 1 mice. Overexpression in N2a-APP17 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.25 | A1309C | destroys its heme structure, resulting in the complete lack of cholesterol 24-hydroxylase activity | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.25 | Homo sapiens | - |
- |
- |
1.14.14.25 | Mus musculus | - |
APP23 mouse | - |
1.14.14.25 | Mus musculus APP23 | - |
APP23 mouse | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.25 | additional information | N2a cell line | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.25 | cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 | - |
Mus musculus | (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.25 | cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 | - |
Homo sapiens | (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.25 | cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 | - |
Mus musculus APP23 | (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.25 | CYP46A1 | - |
Mus musculus |
1.14.14.25 | CYP46A1 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.25 | NADPH | - |
Mus musculus | |
1.14.14.25 | NADPH | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.25 | physiological function | overexpression of human CYP46A1 gene does not modify the expression of endogenous murine Cyp46A1 gene | Mus musculus |
1.14.14.25 | physiological function | selective overexpression of CYP46A1 in neurons can reduce Abeta peptides and amyloid deposits in mouse models of Alzheimer's disease when the overexpression of CYP46A1 is induced before or after the formation of amyloid plaques. Injection of adeno-associated vector encoding CYP46A1 in the cortex and hippocampus of APP23 mice before the onset of amyloid deposits markedly reduces Abeta peptides, amyloid deposits and trimeric oligomers at 12 months of age. Neuronal overexpression of CYP46A1 decreases microgliosis and astrocytosis and improves cognitive deficits in APP23 mice. AAV5-wtCYP46A1 vector injection in the cortex and hippocampus of amyloid precursor protein/presenilin 1 mice after the onset of amyloid deposits also reduces markedly the number of amyloid plaques in the hippocampus, and to a less extent in the cortex, 3 months after the injection | Homo sapiens |