EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.3 | deletion constructs of the ThrRS-dual targeting peptide, ThrRS-dTP, are generated, consisting of amino acid residues 1-50, 1-60, or 24-68, ligated into the pCR Blunt II TOPO vector as a fusion protein with GFP located C-terminally, for a GST-ThrRS-dTP(1-60) construct the vector pGEX-6P-2 is used | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.3 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
6.1.1.3 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.3 | 6600 | - |
ThrRS-dual targeting peptide, determined by SDS-PAGE | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.3 | ATP + L-threonine + tRNAThr | Arabidopsis thaliana | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.3 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.3 | using a RESOURCE S column | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.3 | ATP + L-threonine + tRNAThr | - |
Arabidopsis thaliana | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.3 | Thr-tRNA synthetase | - |
Arabidopsis thaliana |
6.1.1.3 | ThrRS | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.3 | ATP | - |
Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.1.1.3 | physiological function | the final localisation of Thr-tRNA synthetase, expressed as a precursor protein, depends on the dual targeting peptide ThrRS-dTP | Arabidopsis thaliana |