EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.1 | expressed in Escherichia coli BL21(DE3) cells | Mycolicibacterium smegmatis |
2.7.4.1 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.1 | F67A | 84% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.7.4.1 | G69R | 67.1% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.7.4.1 | G74R | 7.6% activity compared to the wild type enzyme, the mutant enzyme cannot interact with nucleoside diphosphate kinase | Mycobacterium tuberculosis |
2.7.4.1 | H115A | 49% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.7.4.1 | H115A/H247A | 6% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.7.4.1 | H247A | 38% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.7.4.1 | K75A | 89% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.4.1 | membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
2.7.4.1 | membrane | - |
Mycobacterium tuberculosis | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.1 | additional information | Mycolicibacterium smegmatis | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
2.7.4.1 | additional information | Mycobacterium tuberculosis | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
2.7.4.1 | additional information | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
2.7.4.1 | additional information | Mycobacterium tuberculosis H37Rv | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.1 | Mycobacterium tuberculosis | - |
- |
- |
2.7.4.1 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
2.7.4.1 | Mycolicibacterium smegmatis | - |
- |
- |
2.7.4.1 | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.1 | Ni2+-NTA agarose column chromatography | Mycolicibacterium smegmatis |
2.7.4.1 | Ni2+-NTA agarose column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.1 | GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycolicibacterium smegmatis | GTP + (phosphate)n | - |
? | |
2.7.4.1 | GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycobacterium tuberculosis | GTP + (phosphate)n | - |
? | |
2.7.4.1 | GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | GTP + (phosphate)n | - |
? | |
2.7.4.1 | GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycobacterium tuberculosis H37Rv | GTP + (phosphate)n | - |
? | |
2.7.4.1 | additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycolicibacterium smegmatis | ? | - |
? | |
2.7.4.1 | additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycobacterium tuberculosis | ? | - |
? | |
2.7.4.1 | additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
2.7.4.1 | additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.1 | polyphosphate kinase 2 | - |
Mycolicibacterium smegmatis |
2.7.4.1 | polyphosphate kinase 2 | - |
Mycobacterium tuberculosis |
2.7.4.1 | PPK2 | - |
Mycolicibacterium smegmatis |
2.7.4.1 | PPK2 | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | GDP | - |
Mycolicibacterium smegmatis | |
2.7.4.1 | GDP | - |
Mycobacterium tuberculosis |