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Literature summary extracted from

  • Klein, S.; Lorenzo, C.; Hoffmann, S.; Walther, J.; Storbeck, S.; Piekarski, T.; Tindall, B.; Wray, V.; Nimtz, M.; Moser, J.
    Adaptation of Pseudomonas aeruginosa to various conditions includes tRNA-dependent formation of alanyl-phosphatidylglycerol (2009), Mol. Microbiol., 71, 551-565.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.11 into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.11 inner membrane
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.11 84000
-
 determined by SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.11 L-alanyl-tRNAAla + phosphatidylglycerol Pseudomonas aeruginosa
-
 tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.11 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.11 from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.11 L-alanyl-tRNAAla + phosphatidylglycerol
-
 Pseudomonas aeruginosa tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.11 A-PG synthase
-
 Pseudomonas aeruginosa
2.3.2.11 A-PGS
-
 Pseudomonas aeruginosa
2.3.2.11 alanylphosphatidylglycerol synthase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.11 35
-
 in vitro activity assay Pseudomonas aeruginosa
2.3.2.11 35
-
 in vivo activity assay Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.11 7.8
-
 in vivo activity assay Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
2.3.2.11 Pseudomonas aeruginosa gene expression is found significantly up-regulated under acidic conditions up