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Literature summary extracted from

  • Prost, L.R.; Daley, M.E.; Bader, M.W.; Klevit, R.E.; Miller, S.I.
    The PhoQ histidine kinases of Salmonella and Pseudomonas spp. are structurally and functionally different: evidence that pH and antimicrobial peptide sensing contribute to mammalian pathogenesis (2008), Mol. Microbiol., 69, 503-519.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 A143G the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
2.7.13.3 A143G the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 D110A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 D110A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
2.7.13.3 D131A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 D131A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
2.7.13.3 D131A the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 D131A the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+ Pseudomonas aeruginosa
2.7.13.3 E115A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
2.7.13.3 E115A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 E133A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 E133A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
2.7.13.3 E77A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
2.7.13.3 E77A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 G139A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
2.7.13.3 G139A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 L137A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 L137A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
2.7.13.3 L158A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 L158A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
2.7.13.3 S145A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 S145A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.13.3 Ca2+
-
 Pseudomonas aeruginosa
2.7.13.3 Ca2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 Mg2+ PhoQ is strongly repressed in divalent cation-replete medium at 10 mM Mg2+ Pseudomonas aeruginosa
2.7.13.3 Mg2+ PhoQ is strongly repressed in divalent cation-replete medium at 10 mM Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.13.3 membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium 16020
-
2.7.13.3 membrane
-
 Pseudomonas aeruginosa 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Pseudomonas aeruginosa
-
-
-
2.7.13.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 ATP + PhoN protein L-histidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + PhoN protein N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + PhoN protein L-histidine
-
 Pseudomonas aeruginosa ADP + PhoN protein N-phospho-L-histidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 PhoQ
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 PhoQ
-
 Pseudomonas aeruginosa
2.7.13.3 sensor histidine kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 sensor histidine kinase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.13.3 ATP
-
 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
2.7.13.3 Salmonella enterica subsp. enterica serovar Typhimurium PhoQ is activated by acidic pH (pH 5.5) up
2.7.13.3 Pseudomonas aeruginosa PhoQ is activated by acidic pH (pH 5.5) up