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Literature summary extracted from

  • Urbonavicius, J.; Auxilien, S.; Walbott, H.; Trachana, K.; Golinelli-Pimpaneau, B.; Brochier-Armanet, C.; Grosjean, H.
    Acquisition of a bacterial RumA-type tRNA(uracil-54, C5)-methyltransferase by Archaea through an ancient horizontal gene transfer (2008), Mol. Microbiol., 67, 323-335.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.35 genes PAB0719 and PAB0760, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Pyrococcus abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.35 additional information
-
 additional information kinetics Pyrococcus abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.35 Fe2+ the enzyme contains an [Fe4S4] cluster, overview Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + tRNA containing uridine at position 54 Pyrococcus abyssi
-
 S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Pyrococcus abyssi
-
 genes PAB0719 and PAB0760
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + tRNA containing uridine at position 54
-
 Pyrococcus abyssi S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
 ?
2.1.1.35 S-adenosyl-L-methionine + tRNA containing uridine at position 54 PAB0719 enzyme or PabTrmU54 displays an identical specificity to eucaryontal homologue TrmA, as it catalyses the in vitro formation of m5U-54 in tRNA Pyrococcus abyssi S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.35 PAB0719 enzyme
-
 Pyrococcus abyssi
2.1.1.35 PabTrmU54
-
 Pyrococcus abyssi
2.1.1.35 RumA
-
 Pyrococcus abyssi
2.1.1.35 RumA-type tRNA(uracil-54, C5)-methyltransferase
-
 Pyrococcus abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 50
-
 assay at Pyrococcus abyssi

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine dependent on Pyrococcus abyssi