Literature summary extracted from

Shin, S.Y.; Kim, I.S.; Kim, Y.H.; Park, H.M.; Lee, J.Y.; Kang, H.G.; Yoon, H.S.
Scavenging reactive oxygen species by rice dehydroascorbate reductase alleviates oxidative stresses in Escherichia coli (2008), Mol. Cells, 26, 616-620.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.5.1	overexpressed in Escherichia coli BL21 (DE3) strain using the pET-28a(+) expression vector	Oryza sativa

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.5.1	Oryza sativa	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.5.1	glutathione + dehydroascorbate	-	Oryza sativa	glutathione disulfide + ascorbate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.5.1	OsDHAR	-	Oryza sativa

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.8.5.1	additional information	-	assay performed at room temperature	Oryza sativa

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.8.5.1	7	-	assay at	Oryza sativa

General Information

EC Number	General Information	Comment	Organism
1.8.5.1	physiological function	OsDHAR transformed Escherichia coli BL21 cells show significantly higher DHAR activity and a lower level of reactive oxygen species than the Escherichia coli cells transformed by an empty vector. The DHAR-overexpressing Escherichia coli strain is more tolerant to oxidant- and heavy metalmediated stress conditions than the control Escherichia coli strain, suggesting that the overexpressed rice DHAR gene effectively functions in a prokaryotic system and provides protection to various oxidative stresses	Oryza sativa

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Release 2023.1 (January 2023)