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Literature summary extracted from

  • Kabuyama, Y.; Litman, E.S.; Templeton, P.D.; Metzner, S.I.; Witze, E.S.; Argast, G.M.; Langer, S.J.; Polvinen, K.; Shellman, Y.; Chan, D.; Shabb, J.B.; Fitzpatrick, J.E.; Resing, K.A.; Sousa, M.C.; Ahn, N.G.
    A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme (2009), Mol. Cell. Proteomics, 8, 2308-2320.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.23 RhoA MRDI is induced by RhoA and mediates RhoA-dependent cell invasion Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.23 into pCMV plasmid for expression in mammalian cells and into pMS356 plasmid for esxpression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.23 C168S inactive mutant Homo sapiens
5.3.1.23 D248A inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.23 RNAi stable knockdown of MRDI is carried out in A-375 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.23 cytoplasm
-
 Homo sapiens 5737
-
5.3.1.23 nucleus
-
 Homo sapiens 5634
-
5.3.1.23 plasma membrane
-
 Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.23 S-methyl-5-thio-alpha-D-ribose 1-phosphate Homo sapiens
-
 S-methyl-5-thio-D-ribulose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.23 by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.23 A-375 cell
-
 Homo sapiens
-
5.3.1.23 FOM71 cell
-
 Homo sapiens
-
5.3.1.23 FOM78 cell
-
 Homo sapiens
-
5.3.1.23 melanocyte cell line
-
 Homo sapiens
-
5.3.1.23 melanoma cell line
-
 Homo sapiens
-
5.3.1.23 NHEM2493 cell
-
 Homo sapiens
-
5.3.1.23 NHEM693 cell
-
 Homo sapiens
-
5.3.1.23 WM-35 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.23 S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 Homo sapiens S-methyl-5-thio-D-ribulose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.23 methylthioribose-1-phosphate isomerase
-
 Homo sapiens
5.3.1.23 MRDI mediator of Rho-dependent invasion Homo sapiens

Expression

EC Number Organism Comment Expression
5.3.1.23 Homo sapiens RhoA induces expression of MRDI up

General Information

EC Number General Information Comment Organism
5.3.1.23 physiological function MRDI is induced in metastatic cells by constitutive RhoA activation and promotes cell invasion Homo sapiens