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Literature summary extracted from

  • van der Knaap, J.A.; Kozhevnikova, E.; Langenberg, K.; Moshkin, Y.M.; Verrijzer, C.P.
    Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes (2010), Mol. Cell. Biol., 30, 736-744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.2 the Ecdysone receptor interacts biochemically and genetically with GMPS/USP7, co-expression of GST-tagged GMPS and USP7 in Escherichia coli strain BL21(DE3) Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.2 C95A mutant defective in glutamine hydrolysis. The phenotype of ectopic coexpression of USP7 witheither S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development Drosophila melanogaster
6.3.5.2 S242L mutant defective in ATP hydrolysis. The phenotype of ectopic coexpression of USP7 with either S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 additional information
-
 additional information binding dynamics at ecdysone target loci, overview Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.5.2 nucleus
-
 Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.2 Mg2+
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.2 additional information Drosophila melanogaster GMP synthetase binds ubiquitin-specific protease 7, USP7, and deubiquitylates histone H2B, the GMPS/USP7 complex cooperates with the Polycomb silencing system through removal of the active ubiquitin mark from histone H2B. GMPS/USP7 binds ecdysone-regulated loci prior to or after hormone signaling. The Ecdysone receptor interacts biochemically and genetically with GMPS/USP7 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Drosophila melanogaster
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 additional information GMP synthetase binds ubiquitin-specific protease 7, USP7, and deubiquitylates histone H2B, the GMPS/USP7 complex cooperates with the Polycomb silencing system through removal of the active ubiquitin mark from histone H2B. GMPS/USP7 binds ecdysone-regulated loci prior to or after hormone signaling. The Ecdysone receptor interacts biochemically and genetically with GMPS/USP7 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.2 GMP synthetase
-
 Drosophila melanogaster
6.3.5.2 GMPS
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.2 ATP
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
6.3.5.2 malfunction GMPS/USP7 complex mutants display severe misregulation of ecdysone target genes Drosophila melanogaster
6.3.5.2 physiological function GMPS is required for its ability to deubiquitylate histone H2B. A key enzyme in the guanine nucleotide biosynthesis pathway is involved in nuclear receptor target silencing suggests that GMPS might provide a relay between metabolic state and developmental gene switching Drosophila melanogaster
6.3.5.2 physiological function GMP synthetase binds ubiquitin-specific protease USP7 and is required for its ability to deubiquitylate histone H2B. Strong cooperation between GMPS and USP7, which is counteracted by the histone H2B ubiquitin ligase BRE1. Loss of either GMPS or USP7 leads to increased levels of histone H2Bub in mutant animals. GMPS/USP7 binds ecdysone-regulated loci and mutants display severe misregulation of ecdysone target genes. Ecdysone receptor EcR interacts biochemically and genetically with GMPS/USP7. Analyses suggest that GMPS/USP7 acts as a transcriptional corepressor Drosophila melanogaster