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Literature summary extracted from

  • Richardson, G.; Ding, H.; Rocheleau, T.; Mayhew, G.; Reddy, E.; Han, Q.; Christensen, B.M.; Li, J.
    An examination of aspartate decarboxylase and glutamate decarboxylase activity in mosquitoes (2009), Mol. Biol. Rep., 37, 3199-3205.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.12 expressed in Escherichia coli Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.12 1.8
-
 L-aspartate in 0.2 M phosphate buffer (pH 7.0) containing 0.1 mM pyridoxal 5'-phosphate, at 25°C Aedes aegypti
4.1.1.15 2.2
-
 L-glutamate in 0.2 M phosphate buffer (pH 7.0) containing 0.1 mM pyridoxal 5'-phosphate, at 25°C Anopheles gambiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.12 110000
-
 gel filtration Aedes aegypti
4.1.1.15 110000
-
 gel filtration Anopheles gambiae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.12 Aedes aegypti
-
-
-
4.1.1.15 Anopheles gambiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.12 chitin bead column chromatography, Mono-Q column chromatography, hydroxylapatite column chromatography, and gel filtration Aedes aegypti
4.1.1.15 Mono-Q column chromatography, hydroxylapatite column chromatography, and gel filtration Anopheles gambiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.12 larva
-
 Aedes aegypti
-
4.1.1.12 pupa
-
 Aedes aegypti
-
4.1.1.15 larva
-
 Anopheles gambiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.12 L-aspartate more than 95% of the substrate is converted to beta-alanine during a 20 min incubation period Aedes aegypti beta-alanine + CO2
-
 ?
4.1.1.12 additional information no activity with L-glutamate Aedes aegypti ?
-
 ?
4.1.1.15 L-glutamate
-
 Anopheles gambiae 4-aminobutanoate + CO2
-
 ?
4.1.1.15 additional information no activity with L-aspartate Anopheles gambiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.12 dimer
-
 Aedes aegypti
4.1.1.15 homodimer gel filtration Anopheles gambiae

Synonyms

EC Number Synonyms Comment Organism
4.1.1.12 ADC
-
 Aedes aegypti
4.1.1.15 GDC
-
 Anopheles gambiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.12 pyridoxal 5'-phosphate
-
 Aedes aegypti
4.1.1.15 pyridoxal 5'-phosphate
-
 Anopheles gambiae