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Literature summary extracted from
- Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations (2009), Mol. Biol. Evol., 26, 2487-2497.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | NAD+ | - |
Erwinia rhapontici |  |
| 3.2.1.86 | NAD+ | - |
Erwinia rhapontici | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | expression in Escherichia coli | Erwinia rhapontici |
| 3.2.1.20 | gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli | Erwinia rhapontici |
| 3.2.1.86 | gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli | Erwinia rhapontici |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | E173S | site-directed mutagenesis | Erwinia rhapontici |
| 3.2.1.20 | E173S | mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy | Erwinia rhapontici |
| 3.2.1.20 | E173S/I174V | site-directed mutagenesis | Erwinia rhapontici |
| 3.2.1.20 | E173S/I174V | mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy | Erwinia rhapontici |
| 3.2.1.20 | I174V | site-directed mutagenesis | Erwinia rhapontici |
| 3.2.1.20 | I174V | mutation with loss of al alpha-glucosidase activity, but the mutant protein exhibits no alpha-galactosidase activtiy | Erwinia rhapontici |
| 3.2.1.86 | E173S | site-directed mutagenesis | Erwinia rhapontici |
| 3.2.1.86 | E173S/I174V | site-directed mutagenesis | Erwinia rhapontici |
| 3.2.1.86 | I174V | site-directed mutagenesis | Erwinia rhapontici |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 1.75 | - |
4-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 2.52 | - |
6-O-alpha-D-glucopyranosyl-D-fructofuranoside | - |
Erwinia rhapontici | |
| 3.2.1.20 | 5.31 | - |
5-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 7.31 | - |
4-O-alpha-D-glucopyranosyl-D-sorbitol | - |
Erwinia rhapontici | |
| 3.2.1.20 | 8.58 | - |
4-O-alpha-D-glucopyranosyl-D-glucopyranose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 10.04 | - |
3-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 22.11 | - |
1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside | - |
Erwinia rhapontici | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.20 | soluble | - |
Erwinia rhapontici | - |
- |
| 3.2.1.86 | soluble | - |
Erwinia rhapontici | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | Mn2+ | - |
Erwinia rhapontici | |
| 3.2.1.86 | Mn2+ | - |
Erwinia rhapontici | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 50000 | - |
SDS-PAGE | Erwinia rhapontici |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Erwinia rhapontici | - |
- |
- |
| 3.2.1.20 | Erwinia rhapontici | Q9AI65 | strain DSM448, gene palH | - |
| 3.2.1.20 | Erwinia rhapontici DSM 448 | Q9AI65 | strain DSM448, gene palH | - |
| 3.2.1.86 | Erwinia rhapontici | Q9AI65 | strain DSM448, gene palH | - |
| 3.2.1.86 | Erwinia rhapontici DSM 448 | Q9AI65 | strain DSM448, gene palH | - |
| 3.2.1.122 | Symbiobacterium thermophilum IAM 14863 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | anion exchange chormatography and gel filtration chromatography | Erwinia rhapontici |
| 3.2.1.20 | recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration | Erwinia rhapontici |
| 3.2.1.86 | recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration | Erwinia rhapontici |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | 1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.20 | 3-O-alpha-D-glucopyranosyl-D-fructose + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | assay at pH 7.5, 37° | Erwinia rhapontici | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-O-alpha-D-glucopyranosyl-D-fructose + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.20 | 4-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O | - |
Erwinia rhapontici | alpha-D-glucose + alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-O-alpha-D-glucopyranosyl-D-sorbitol + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-sorbitol | - |
? | |
| 3.2.1.20 | 5-O-alpha-D-glucopyranosyl-D-fructose + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.20 | 6-O-alpha-D-glucopyranosyl-D-fructofuranoside + H2O | - |
Erwinia rhapontici | alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.20 | additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici | ? | - |
? | |
| 3.2.1.20 | additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici DSM 448 | ? | - |
? | |
| 3.2.1.86 | additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici | ? | - |
? | |
| 3.2.1.86 | additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici DSM 448 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | 6-phospho-alpha-glucosidase | - |
Erwinia rhapontici |
| 3.2.1.20 | alpha-glucosid-specific GH4 enzyme | - |
Erwinia rhapontici |
| 3.2.1.20 | alpha-glucosidase | - |
Erwinia rhapontici |
| 3.2.1.20 | CHEI | - |
Erwinia rhapontici |
| 3.2.1.20 | More | the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site | Erwinia rhapontici |
| 3.2.1.20 | PalH | - |
Erwinia rhapontici |
| 3.2.1.20 | phospho-alpha-glucosidase | - |
Erwinia rhapontici |
| 3.2.1.86 | 6-phospho-alpha-glucosidase | - |
Erwinia rhapontici |
| 3.2.1.86 | More | the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site | Erwinia rhapontici |
| 3.2.1.86 | PalH | - |
Erwinia rhapontici |
| 3.2.1.86 | phospho-alpha-glucosidase | - |
Erwinia rhapontici |
| 3.2.1.122 | 6-phospho-alpha-glucosidase | - |
Symbiobacterium thermophilum IAM 14863 |
| 3.2.1.122 | CDMP | - |
Symbiobacterium thermophilum IAM 14863 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 25 | - |
assay at | Erwinia rhapontici |
| 3.2.1.20 | 37 | - |
assay at | Erwinia rhapontici |
| 3.2.1.86 | 25 | - |
assay at | Erwinia rhapontici |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.2 | - |
4-O-alpha-D-glucopyranosyl-D-sorbitol | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.27 | - |
1-O-alpha-D-glucopyranosyl-beta-D-fructofuranoside | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.37 | - |
5-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.38 | - |
3-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.45 | - |
4-O-alpha-D-glucopyranosyl-D-fructose | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.58 | - |
6-O-alpha-D-glucopyranosyl-D-fructofuranoside | - |
Erwinia rhapontici | |
| 3.2.1.20 | 0.85 | - |
4-O-alpha-D-glucopyranosyl-D-glucopyranose | - |
Erwinia rhapontici | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 7.5 | - |
assay at | Erwinia rhapontici |
| 3.2.1.86 | 7.5 | - |
assay at | Erwinia rhapontici |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | NAD+ | - |
Erwinia rhapontici | |
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Release 2023.1 (January 2023)
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