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Literature summary extracted from

  • Husain, A.; Jeelani, G.; Sato, D.; Ali, V.; Nozaki, T.
    Characterization of two isotypes of L-threonine dehydratase from Entamoeba histolytica (2010), Mol. Biochem. Parasitol., 170, 100-104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 additional information insensitive to the allosteric activation by AMP or CMP Entamoeba histolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.17 His-tagged version expressed in Escherichia coli BL21(DE3) Entamoeba histolytica
4.3.1.19
-
 Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.17 2-oxobutyrate 20 mM: 49.3% inhibition, not reversed by 5 mM AMP Entamoeba histolytica
4.3.1.17 ATP
-
 Entamoeba histolytica
4.3.1.17 glyoxylate 20 mM: 54.1% inhibition, not reversed by 5 mM AMP Entamoeba histolytica
4.3.1.17 L-cysteine competitive Entamoeba histolytica
4.3.1.17 pyruvate 20 mM: 45.3% inhibition, not reversed by 5 mM AMP Entamoeba histolytica
4.3.1.19 2-oxobutanoate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica
4.3.1.19 glyoxylate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica
4.3.1.19 L-Cys competitive Entamoeba histolytica
4.3.1.19 pyruvate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 7.3
-
 L-threonine
-
 Entamoeba histolytica
4.3.1.17 13.5
-
 L-serine
-
 Entamoeba histolytica
4.3.1.19 7.3
-
 L-Thr pH 9.0, 37°C Entamoeba histolytica
4.3.1.19 13.5
-
 L-Ser pH 9.0, 37°C Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 45000
-
 x * 45000, calculated Entamoeba histolytica
4.3.1.19 45000
-
 x * 45000, calculated Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Entamoeba histolytica B1N2N4
-
-
4.3.1.19 Entamoeba histolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.17 immobilized metal ion affinity chromatography (Ni2+) Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-serine
-
 Entamoeba histolytica pyruvate + NH3
-
 ?
4.3.1.17 L-threonine
-
 Entamoeba histolytica 2-oxobutyrate + NH3
-
 ?
4.3.1.19 L-Ser
-
 Entamoeba histolytica pyruvate + NH3
-
 ir
4.3.1.19 L-Thr
-
 Entamoeba histolytica 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.17 ? x * 45000, calculated Entamoeba histolytica
4.3.1.19 ? x * 45000, calculated Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
4.3.1.17 L-serine dehydratase
-
 Entamoeba histolytica
4.3.1.17 L-threonine dehydratase
-
 Entamoeba histolytica
4.3.1.19 TD1
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.17 3
-
 L-serine
-
 Entamoeba histolytica
4.3.1.17 5.2
-
 L-threonine
-
 Entamoeba histolytica
4.3.1.19 3
-
 L-Ser pH 9.0, 37°C Entamoeba histolytica
4.3.1.19 5.2
-
 L-Thr pH 9.0, 37°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.17 8.5 9
-
 Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.17 pyridoxal 5'-phosphate
-
 Entamoeba histolytica
4.3.1.19 pyridoxal 5'-phosphate
-
 Entamoeba histolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.17 1.1
-
 L-cysteine substrate L-serine Entamoeba histolytica
4.3.1.17 2.2
-
 L-cysteine substrate L-threonine Entamoeba histolytica
4.3.1.19 1.1
-
 L-Cys substrate L-Ser, pH 9.0, 37°C Entamoeba histolytica
4.3.1.19 2.2
-
 L-Cys substrate L-Thr, pH 9.0, 37°C Entamoeba histolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.3.1.17 Entamoeba histolytica calculated
-
 6.68
4.3.1.19 Entamoeba histolytica calculated
-
 6.7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.1.17 10
-
-
 Entamoeba histolytica ATP

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.17 0.2
-
 L-serine
-
 Entamoeba histolytica
4.3.1.17 0.7
-
 L-threonine
-
 Entamoeba histolytica
4.3.1.19 0.2
-
 L-Ser pH 9.0, 37°C Entamoeba histolytica
4.3.1.19 0.7
-
 L-Thr pH 9.0, 37°C Entamoeba histolytica