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Literature summary extracted from

  • Pang, C.K.; Hunter, J.H.; Gujjar, R.; Podutoori, R.; Bowman, J.; Mudeppa, D.G.; Rathod, P.K.
    Catalytic and ligand-binding characteristics of Plasmodium falciparum serine hydroxymethyltransferase (2009), Mol. Biochem. Parasitol., 168, 74-83.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) and GS245(DE3) cells Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 1843U89
-
 Plasmodium falciparum
2.1.2.1 AG331
-
 Plasmodium falciparum
2.1.2.1 AG337
-
 Plasmodium falciparum
2.1.2.1 D1694
-
 Plasmodium falciparum
2.1.2.1 GR1
-
 Plasmodium falciparum
2.1.2.1 methotrexate
-
 Plasmodium falciparum
2.1.2.1 permetrexed
-
 Plasmodium falciparum
2.1.2.1 pyrimethamine
-
 Plasmodium falciparum
2.1.2.1 WR99210
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.7
-
 L-serine in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum
2.1.2.1 3.4
-
 tetrahydrofolate in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 55000
-
 2 * 55000, His-tagged enzyme, SDS-PAGE Plasmodium falciparum
2.1.2.1 81500
-
 calculated molecular weight Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 the His-tagged enzyme is purified by His GraviTrap column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Plasmodium falciparum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homodimer 2 * 55000, His-tagged enzyme, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Plasmodium falciparum
2.1.2.1 SHMT
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.16
-
 L-serine in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate dependent on, the apo enzyme has some enzyme activity in the absence of pyridoxal 5'-phosphate but this activity is only slightly above background and can be due to incomplete removal of pyridoxal 5'-phosphate Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.2.1 0.125
-
 in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum WR99210
2.1.2.1 0.15
-
 in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum 1843U89
2.1.2.1 0.15
-
 in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum GR1
2.1.2.1 0.15
-
 in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum AG331
2.1.2.1 0.2
-
 in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum AG337
2.1.2.1 0.5
-
 IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum methotrexate
2.1.2.1 0.5
-
 IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum pyrimethamine
2.1.2.1 0.5
-
 IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum permetrexed
2.1.2.1 0.5
-
 IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum D1694