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Literature summary extracted from

  • Yoshida, Y.; Sasaki, T.; Ito, S.; Tamura, H.; Kunimatsu, K.; Kato, H.
    Identification and molecular characterization of tryptophanase encoded by tnaA in Porphyromonas gingivalis (2009), Microbiology, 155, 968-978.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.1 expressed in Escherichia coli BL21(DE3) cells Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.1 0.09
-
 L-Trp purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 0.2
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 0.26
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 2.04
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.1 additional information TnaA is not affected by K+ or Na+ Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 52500
-
 calculated from amino acid sequence Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Porphyromonas gingivalis
-
-
-
4.1.99.1 Porphyromonas gingivalis W83
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1 glutathione-Sepharose column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-Trp + H2O
-
 Porphyromonas gingivalis indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Porphyromonas gingivalis W83 indole + pyruvate + NH4+
-
 ?
4.1.99.1 additional information tryptophanase does not degrade L-alanine, L-serine or L-cysteine Porphyromonas gingivalis ?
-
 ?
4.1.99.1 additional information tryptophanase does not degrade L-alanine, L-serine or L-cysteine Porphyromonas gingivalis W83 ?
-
 ?
4.1.99.1 S-ethyl-L-cysteine + H2O
-
 Porphyromonas gingivalis ?
-
 ?
4.1.99.1 S-ethyl-L-cysteine + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
4.1.99.1 S-methyl-L-cysteine + H2O
-
 Porphyromonas gingivalis ?
-
 ?
4.1.99.1 S-methyl-L-cysteine + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.1 TnaA
-
 Porphyromonas gingivalis
4.1.99.1 tryptophan indole-lyase
-
 Porphyromonas gingivalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.99.1 40 70 the enzyme is stable up to 40°C, but only 40% of the activity remains at 60°C, the enzyme is completely inactivated at temperatures above 70°C Porphyromonas gingivalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.1 0.24
-
 L-Trp purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 0.5
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 1.37
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 1.87
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.1 7.5
-
-
 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.1 pyridoxal 5'-phosphate
-
 Porphyromonas gingivalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.99.1 0.25
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 6.87
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
4.1.99.1 7.15
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis