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Literature summary extracted from

  • Lindsay, A.M.; Zhang, M.; Mitchell, Z.; Holden, M.T.; Waller, A.S.; Sutcliffe, I.C.; Black, G.W.
    The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection (2009), Microbiology, 155, 443-449.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.1 His-tagged version expressed in Escherichia coli BL21(DE3) Streptococcus equi subsp. equi 4047

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.1 D171A catalytic mutant, 8% activity Streptococcus equi subsp. equi 4047
4.2.2.1 Y183A catalytic mutant, 13% activity Streptococcus equi subsp. equi 4047

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.1 additional information not inhibited by ascorbic acid at concentration up to 20 mM Streptococcus equi subsp. equi 4047

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.1 0.71
-
 hyaluronic acid concentration expressed as the concentration of its disaccharide unit (equivalent to 0.29 mg/ml) Streptococcus equi subsp. equi 4047

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.1 additional information no requirement for any specific divalent ion tested Streptococcus equi subsp. equi 4047

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.1 42000
-
 x * 42000, SDS-PAGE Streptococcus equi subsp. equi 4047

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus equi subsp. equi 4047 C0M9W4 prophage encoded
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.1
-
 Streptococcus equi subsp. equi 4047

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronic acid no activity against chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate Streptococcus equi subsp. equi 4047 ? end-products are 4,5-unsaturated hyalurono-hexasaccharide and 4,5-unsaturated hyalurono-octasaccharide, with small amounts of 4,5-unsaturated hyalurono-tetrasaccharide, 4,5-unsaturated hyalurono-decasaccharide and longer oligosaccharides, early time points shows that a wide range of 4,5-unsaturated oligosaccharides are produced, indicating an endo-acting mode of digestion ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 ? x * 42000, SDS-PAGE Streptococcus equi subsp. equi 4047

Synonyms

EC Number Synonyms Comment Organism
4.2.2.1 hyaluronate lyase
-
 Streptococcus equi subsp. equi 4047
4.2.2.1 protein SEQ2045
-
 Streptococcus equi subsp. equi 4047

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.1 37
-
 50% activity at 44°C Streptococcus equi subsp. equi 4047

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.1 7.61
-
 hyaluronic acid
-
 Streptococcus equi subsp. equi 4047

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.1 7
-
-
 Streptococcus equi subsp. equi 4047

General Information

EC Number General Information Comment Organism
4.2.2.1 physiological function produced during infection of horses Streptococcus equi subsp. equi 4047