BRENDA - Enzyme Database

Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host

Candela, M.; Biagi, E.; Centanni, M.; Turroni, S.; Vici, M.; Musiani, F.; Vitali, B.; Bergmann, S.; Hammerschmidt, S.; Brigidi, P.; Microbiology 155, 3294-3303 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 Star(DE3) cells
Bifidobacterium animalis subsp. lactis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cell surface
-
Bifidobacterium animalis subsp. lactis
9986
-
4.2.1.11
cell surface
-
Bifidobacterium bifidum
9986
-
4.2.1.11
cell surface
-
Bifidobacterium breve
9986
-
4.2.1.11
cell surface
-
Bifidobacterium longum
9986
-
4.2.1.11
cytoplasm
-
Bifidobacterium animalis subsp. lactis
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium bifidum
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium breve
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium longum
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
SDS-PAGE
Bifidobacterium animalis subsp. lactis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium bifidum
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium breve
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium longum
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium animalis subsp. lactis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium animalis subsp. lactis BI07
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium bifidum S16
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium breve BBSF
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Bifidobacterium animalis subsp. lactis
Q45RT9
-
-
4.2.1.11
Bifidobacterium animalis subsp. lactis BI07
Q45RT9
-
-
4.2.1.11
Bifidobacterium bifidum
-
-
-
4.2.1.11
Bifidobacterium bifidum S16
-
-
-
4.2.1.11
Bifidobacterium breve
-
-
-
4.2.1.11
Bifidobacterium breve BBSF
-
-
-
4.2.1.11
Bifidobacterium longum
-
strain S123
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA resin column chromatography
Bifidobacterium animalis subsp. lactis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium bifidum
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium breve
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium longum
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium animalis subsp. lactis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium animalis subsp. lactis BI07
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium bifidum S16
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium breve BBSF
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium bifidum
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium breve
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium longum
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium animalis subsp. lactis
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium animalis subsp. lactis BI07
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium bifidum S16
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium breve BBSF
?
-
-
-
-
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Bifidobacterium animalis subsp. lactis
isoelectric focusing
-
4.7
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 Star(DE3) cells
Bifidobacterium animalis subsp. lactis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cell surface
-
Bifidobacterium animalis subsp. lactis
9986
-
4.2.1.11
cell surface
-
Bifidobacterium bifidum
9986
-
4.2.1.11
cell surface
-
Bifidobacterium breve
9986
-
4.2.1.11
cell surface
-
Bifidobacterium longum
9986
-
4.2.1.11
cytoplasm
-
Bifidobacterium animalis subsp. lactis
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium bifidum
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium breve
5737
-
4.2.1.11
cytoplasm
-
Bifidobacterium longum
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
SDS-PAGE
Bifidobacterium animalis subsp. lactis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium bifidum
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium breve
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium longum
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium animalis subsp. lactis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium animalis subsp. lactis BI07
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium bifidum S16
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Bifidobacterium breve BBSF
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA resin column chromatography
Bifidobacterium animalis subsp. lactis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium bifidum
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium breve
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium longum
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium animalis subsp. lactis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium animalis subsp. lactis BI07
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium bifidum S16
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
705569
Bifidobacterium breve BBSF
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium bifidum
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium breve
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium longum
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium animalis subsp. lactis
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium animalis subsp. lactis BI07
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium bifidum S16
?
-
-
-
-
4.2.1.11
additional information
His-tagged recombinant enolase protein shows a high affinity for human plasminogen
705569
Bifidobacterium breve BBSF
?
-
-
-
-
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Bifidobacterium animalis subsp. lactis
isoelectric focusing
-
4.7
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium animalis subsp. lactis
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium bifidum
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium breve
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium longum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium animalis subsp. lactis
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium bifidum
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium breve
4.2.1.11
physiological function
enolase is a conserved putative human plasminogen receptor in Bifidobacterium
Bifidobacterium longum