Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Deveryshetty, J.; Phale, P.S.
    Biodegradation of phenanthrene by Pseudomonas sp. strain PPD: purification and characterization of 1-hydroxy-2-naphthoic acid dioxygenase (2009), Microbiology, 155, 3083-3091.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.38 the enzyme is very unstable in the cell-free extract and requires Fe(II) (0.1 mM), DTT (2 mM) and ethanol (10%) for stability Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.38 1-hydroxy-2-naphthoic acid substrate inhibition Pseudomonas sp.
1.13.11.38 3-Hydroxy-2-naphthoic acid competitive inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.38 0.0135
-
 1-hydroxy-2-naphthoate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.38 Fe2+
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.38 39000
-
 SDS-PAGE under denaturing conditions, 4 * 39000 Pseudomonas sp.
1.13.11.38 160000
-
 Sephacryl S-200-HR gel-filtration chromatography; SDS-PAGE under denaturing conditions, homotetramer, subunit molecular mass of about 39000 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.38 1-hydroxy-2-naphthoic acid + O2 Pseudomonas sp.
-
 2-carboxybenzylpyruvic acid
-
 ?
1.13.11.38 additional information Pseudomonas sp. 1-hydroxy-2-naphthoic acid dioxygenase shows no activity with gentisic acid, salicylic acid and other hydroxynaphthoic acids ?
-
 ?
1.13.11.38 additional information Pseudomonas sp. Pseudomonas sp. strain PPD can metabolize phenanthrene as the sole source of carbon and energy via the phthalic acid route ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.38 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.38 to homogeneity using a 3-hydroxy-2-naphthoic acid (3-H2NA)-affinity matrix, DEAE-anion-exchange chromatography Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.38 culture condition:phenanthrene-grown cell
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.38 4.9
-
 cell-free extract Pseudomonas sp.
1.13.11.38 11.2
-
 heat treatment (60°C) Pseudomonas sp.
1.13.11.38 29
-
 anion-exchange chromatography Pseudomonas sp.
1.13.11.38 53
-
 3-H2NA-affinity chromatography Pseudomonas sp.
1.13.11.38 61.5
-
 after all purification steps from Pseudomonas sp. PPD cells grown on phenanthrene Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.38 1-hydroxy-2-naphthoate + O2
-
 Pseudomonas sp. (3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 r
1.13.11.38 1-hydroxy-2-naphthoic acid + O2
-
 Pseudomonas sp. 2-carboxybenzylpyruvic acid
-
 ?
1.13.11.38 additional information 1-hydroxy-2-naphthoic acid dioxygenase shows no activity with gentisic acid, salicylic acid and other hydroxynaphthoic acids Pseudomonas sp. ?
-
 ?
1.13.11.38 additional information Pseudomonas sp. strain PPD can metabolize phenanthrene as the sole source of carbon and energy via the phthalic acid route Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.38 tetramer SDS-PAGE under denaturing conditions, 4 * 39000 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.38 1-HNDO
-
 Pseudomonas sp.
1.13.11.38 1-hydroxy-2-naphthoic acid dioxygenase
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.38 60
-
 heat stability for 20 min at 60°C Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.38 70.4
-
 1-hydroxy-2-naphthoate
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.38 7.5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.38 6 9
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.38 0.024
-
 3-Hydroxy-2-naphthoic acid competitive inhibition Pseudomonas sp.
1.13.11.38 0.116
-
 1-hydroxy-2-naphthoic acid substrate inhibition Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.13.11.38 metabolism metabolic pathway for degradation of phenanthrene in Pseudomonas sp. strain PPD Pseudomonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.38 5185
-
 2-Hydroxy-1-naphthoic acid
-
 Pseudomonas sp.