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Literature summary extracted from

  • Avila, M.; Jaquet, M.; Moine, D.; Requena, T.; Pelaez, C.; Arigoni, F.; Jankovic, I.
    Physiological and biochemical characterization of the two alpha-L-rhamnosidases of Lactobacillus plantarum NCC245 (2009), Microbiology, 155, 2739-2749.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.40 EDTA a slight activating influence on isoform RhaB1 is observed for EDTA at 10 mM Lactiplantibacillus plantarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expressed in Escherichia coli BL21 S1 cells Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 1,10-phenanthroline less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB1 decreases by 50%; less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB2 decreases by 50% Lactiplantibacillus plantarum
3.2.1.40 Cu2+
-
 Lactiplantibacillus plantarum
3.2.1.40 Fe2+
-
 Lactiplantibacillus plantarum
3.2.1.40 L-rhamnose isoform RhaB1 is slightly inhibited by L-rhamnose, showing noncompetitive inhibition Lactiplantibacillus plantarum
3.2.1.40 Mn2+
-
 Lactiplantibacillus plantarum
3.2.1.40 additional information dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity Lactiplantibacillus plantarum
3.2.1.40 phosphoramidon isoform RhaB1 shows 23.8% residual activity at 1 mM; isoform RhaB2 shows 42.5% residual activity at 1 mM Lactiplantibacillus plantarum
3.2.1.40 polyhexamethylene biguanide 1 mM reduces the activity of isoform RhaB1 to 10.9% and isoform RhaB2 to 36.0% residual activity; 1 mM reduces the activity of isoform RhaB1 to 10.9% residual activity Lactiplantibacillus plantarum
3.2.1.40 SDS SDS produces partial inactivation; SDS produces partial inactivation Lactiplantibacillus plantarum
3.2.1.40 thiorphan less effective inhibitor; less effective inhibitor Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.51
-
 4-nitrophenyl alpha-L-rhamnopyranoside isoform RhaB2 Lactiplantibacillus plantarum
3.2.1.40 0.57
-
 4-nitrophenyl alpha-L-rhamnopyranoside isoform RhaB1 Lactiplantibacillus plantarum
3.2.1.40 0.78
-
 hesperidin isoform RhaB2 Lactiplantibacillus plantarum
3.2.1.40 1.06
-
 rutin isoform RhaB2 Lactiplantibacillus plantarum
3.2.1.40 1.77
-
 rutin isoform RhaB1 Lactiplantibacillus plantarum
3.2.1.40 3.39
-
 hesperidin isoform RhaB1 Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.40 Ca2+
-
 Lactiplantibacillus plantarum
3.2.1.40 Co2+
-
 Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Lactiplantibacillus plantarum C4PG45 isoform RhaB2
-
3.2.1.40 Lactiplantibacillus plantarum C4PG47 isoform RhaB1
-
3.2.1.40 Lactiplantibacillus plantarum NCC245 C4PG45 isoform RhaB2
-
3.2.1.40 Lactiplantibacillus plantarum NCC245 C4PG47 isoform RhaB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 HiTrap chelating column chromatography, gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Lactiplantibacillus plantarum 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Lactiplantibacillus plantarum NCC245 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnoside + H2O
-
 Lactiplantibacillus plantarum alpha-L-rhamnose + 4-nitrophenol
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnoside + H2O
-
 Lactiplantibacillus plantarum NCC245 alpha-L-rhamnose + 4-nitrophenol
-
 ?
3.2.1.40 hesperidin + H2O 11.2% relative activity compared to rutin Lactiplantibacillus plantarum hesperetin 7-O-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O isoform RhaB2 exhibits highest activity against hesperidin Lactiplantibacillus plantarum hesperetin 7-O-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O isoform RhaB2 exhibits highest activity against hesperidin Lactiplantibacillus plantarum NCC245 hesperetin 7-O-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O 11.2% relative activity compared to rutin Lactiplantibacillus plantarum NCC245 hesperetin 7-O-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 additional information no activity is detected towards the alpha-1,2 linkage in naringin Lactiplantibacillus plantarum ?
-
 ?
3.2.1.40 additional information no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine Lactiplantibacillus plantarum ?
-
 ?
3.2.1.40 additional information no activity is detected towards the alpha-1,2 linkage in naringin Lactiplantibacillus plantarum NCC245 ?
-
 ?
3.2.1.40 additional information no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine Lactiplantibacillus plantarum NCC245 ?
-
 ?
3.2.1.40 proscillaridin A + H2O 0.4% relative activity compared to hesperidin Lactiplantibacillus plantarum ?
-
 ?
3.2.1.40 proscillaridin A + H2O 4.5% relative activity compared to rutin Lactiplantibacillus plantarum ?
-
 ?
3.2.1.40 rutin + H2O 10.1% relative activity compared to hesperidin Lactiplantibacillus plantarum isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O isoform RhaB1 exhibits highest activity against rutin Lactiplantibacillus plantarum isoquercitrin + alpha-L-rhamnose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 RhaB1 isoform Lactiplantibacillus plantarum
3.2.1.40 RhaB2 isoform Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 50
-
 isoform RhaB1 Lactiplantibacillus plantarum
3.2.1.40 60
-
 isoform RhaB2 Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 30 60 the enzyme shows relative activities over 64% from 30 to 60°C Lactiplantibacillus plantarum
3.2.1.40 50 70 isoform RhaB2 is most active at temperatures between 50 and 70°C, still showing 70% relative activity at 70°C Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 55 65 isoform RhaB2 retains 43% and 25% activity after 5 h incubation at 55°C and 65°C, respectively Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5
-
 isoform RhaB2 Lactiplantibacillus plantarum
3.2.1.40 7
-
 isoform RhaB1 Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 4.4 5.5 isoform RhaB2 maintains over 60% of its activity in the pH range from 4.4 to 5.5 Lactiplantibacillus plantarum
3.2.1.40 5 7.5 isoform RhaB1 maintains over 60% of its activity in the pH range from 5.0 to 7.5 Lactiplantibacillus plantarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 3 9 isoform RhaB1 remains active over a broad range of pH values, showing 90-100% remaining relative activities from pH 4.0 to 7.5, and over 40% at pH 3 and 9, after 5 h at 37°C Lactiplantibacillus plantarum
3.2.1.40 3 9 the stability of isoform RhaB2 is lower than that of isoform RhaB1, with 80-85% enzymatic activity from pH 5.0 to 7.0, and only 17% and 12% at pH 3.0 and 9.0, respectively Lactiplantibacillus plantarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 19.88
-
 L-rhamnose isoform RhaB1 Lactiplantibacillus plantarum

Expression

EC Number Organism Comment Expression
3.2.1.40 Lactiplantibacillus plantarum the enzyme activity is downregulated by D-glucose down
3.2.1.40 Lactiplantibacillus plantarum the enzyme activity is upregulated by L-rhamnose up