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Literature summary extracted from

  • Furmanek-Blaszk, B.; Boratynski, R.; Zolcinska, N.; Sektas, M.
    M1.Mboll and M2.Mboll type IIS methyltransferases: different specificities, the same target (2009), Microbiology, 155, 1111-1121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli ER2566 cells Moraxella bovis
2.1.1.113 gene mboIIR, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli using the constitutive promoter, PtetA Moraxella bovis
2.1.1.113 gene ncuIM2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Moraxella cuniculi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.72 Mg2+ Mg2+ inhibits methylation activity Moraxella bovis
2.1.1.113 Ca2+
-
 Moraxella bovis
2.1.1.113 Ca2+ slight inhibition Moraxella cuniculi
2.1.1.113 Mg2+
-
 Moraxella bovis
2.1.1.113 Mg2+ slight inhibition Moraxella cuniculi
2.1.1.113 Mn2+
-
 Moraxella bovis
2.1.1.113 Mn2+ strong inhibition Moraxella cuniculi
2.1.1.113 Zn2+
-
 Moraxella bovis
2.1.1.113 Zn2+ strong inhibition Moraxella cuniculi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.113 30000
-
 1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi
2.1.1.113 30000
-
 1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis
2.1.1.113 32111
-
 1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis
2.1.1.113 32676
-
 1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 additional information Moraxella bovis no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Moraxella bovis M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine Moraxella bovis
-
 S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine Moraxella cuniculi
-
 S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Moraxella bovis
-
 strain ATCC 10900
-
2.1.1.113 Moraxella bovis
-
 strain ATCC 10900, gene mboIIR
-
2.1.1.113 Moraxella cuniculi
-
 strain ATCC 14688, gene ncuIM2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 partial purification using phosphocellulose column chromatography, hydroxylapatite column chromatography, CM-Sephadex gel filtration, and heparin agarose column chromatography Moraxella bovis
2.1.1.113 recombinant M2.MboII 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydroophobic interaction and heparin affinity chromatography, from Escherichia coli Moraxella bovis
2.1.1.113 recombinant M2.NcuI 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydrophobic interaction and heparin affinity chromatography, from Escherichia coli Moraxella cuniculi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.113 3200
-
 purified recombinant enzyme Moraxella bovis
2.1.1.113 3200
-
 purified recombinant enzyme Moraxella cuniculi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA Moraxella bovis ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine Moraxella bovis S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine
-
 Moraxella bovis S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine
-
 Moraxella cuniculi S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine substrate is pNH20 plasmid DNA. M2.MboII modifies the internal cytosine in the recognition sequence 39-CTTCT-59, yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination and analysis of the methylation pattern of M2.MboII, overview Moraxella bovis S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine substrate is pNH20 plasmid DNA. M2.NcuI modifies the internal cytosine in the recognition sequence yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination of the methylation position, overview Moraxella cuniculi S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.113 monomer 1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi
2.1.1.113 monomer 1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 adenine N6-methyltransferase
-
 Moraxella bovis
2.1.1.72 M1.MboII a type IIS methyltransferase Moraxella bovis
2.1.1.113 M2.MboII
-
 Moraxella bovis
2.1.1.113 M2.NcuI
-
 Moraxella cuniculi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.113 37
-
 assay at Moraxella bovis
2.1.1.113 37
-
 assay at Moraxella cuniculi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.113 7 8
-
 Moraxella bovis
2.1.1.113 7
-
 assay at Moraxella cuniculi

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.113 S-adenosyl-L-methionine
-
 Moraxella bovis
2.1.1.113 S-adenosyl-L-methionine
-
 Moraxella cuniculi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.113 0.4
-
 pH 7.0, 37°C, recombinant enzyme Moraxella bovis Mn2+
2.1.1.113 0.4
-
 pH 7.0, 37°C, recombinant enzyme Moraxella bovis Zn2+
2.1.1.113 4
-
 pH 7.0, 37°C, recombinant enzyme Moraxella bovis Ca2+
2.1.1.113 5
-
 pH 7.0, 37°C, recombinant enzyme Moraxella bovis Mg2+

General Information

EC Number General Information Comment Organism
2.1.1.113 metabolism the enzyme is part of the MboII restriction-modification, R-M, system of Moraxella bovis strain ATCC 10900 consisting of a restriction endonuclease gene and two methyltransferase genes Moraxella bovis
2.1.1.113 metabolism the enzyme is part of the Ncul restriction-modification, R-M, system consisting of a restriction endonuclease gene and two methyltransferase genes Moraxella cuniculi