Literature summary extracted from

Kim, Y.M.; Yeon, M.J.; Choi, N.S.; Chang, Y.H.; Jung, M.Y.; Song, J.J.; Kim, J.S.
Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001 (2010), Microbiol. Res., 165, 384-391.

Application

EC Number	Application	Comment	Organism
2.4.1.5	synthesis	the enzyme is useful for production of L-ascorbic acid 2-glucoside for use as an antioxidant in industrial applications	Leuconostoc lactis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.5	DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged glucansucrase in Escherichia coli	Leuconostoc lactis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.1.5	1.8	-	sucrose	pH 5.0, 30°C, recombinant enzyme	Leuconostoc lactis

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.5	165000	-	x * 165000, recombinant enzyme, SDS-PAGE	Leuconostoc lactis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.5	Leuconostoc lactis	C7DT60	isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food	-
2.4.1.5	Leuconostoc lactis EG001	C7DT60	isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.5	recombinant His-tagged glucansucrase 2.4fold from Escherichia coli by nickel affinity chromatography	Leuconostoc lactis

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.1.5	126	-	purified recombinant enzyme	Leuconostoc lactis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.5	sucrose + L-ascorbic acid	recombinant glucansucrase	Leuconostoc lactis	D-fructose + L-ascorbic acid 2-glucoside	the glycosylated product has the potential as an antioxidant in industrial applications	?
2.4.1.5	sucrose + L-ascorbic acid	recombinant glucansucrase	Leuconostoc lactis EG001	D-fructose + L-ascorbic acid 2-glucoside	the glycosylated product has the potential as an antioxidant in industrial applications	?
2.4.1.5	sucrose + [(1,6)-alpha-D-glucosyl]n	-	Leuconostoc lactis	D-fructose + [(1,6)-alpha-D-glucosyl]n+1	-	?
2.4.1.5	sucrose + [(1,6)-alpha-D-glucosyl]n	-	Leuconostoc lactis EG001	D-fructose + [(1,6)-alpha-D-glucosyl]n+1	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.1.5	?	x * 165000, recombinant enzyme, SDS-PAGE	Leuconostoc lactis

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.5	glucansucrase	-	Leuconostoc lactis

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.4.1.5	30	-	-	Leuconostoc lactis

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.4.1.5	10	42	activity range of the recombinant enzyme, profile, overview	Leuconostoc lactis

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.1.5	5	-	-	Leuconostoc lactis

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
2.4.1.5	3.5	6.8	activity range of the recombinant enzyme, profile, overview	Leuconostoc lactis

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Release 2023.1 (January 2023)