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Literature summary extracted from
- Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei (2009), Microb. Cell Fact., 8, 34.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | expressed in Escherichia coli BL21(DE3) cells | Enterobacter hormaechei |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | beta-mercaptoethanol | complete inhibition at 1 mM | Enterobacter hormaechei |  |
| 5.4.99.16 | Cu2+ | 52% residual activity at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | Fe2+ | 92% residual activity at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | Hg2+ | 57% residual activity at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | additional information | TreS activity is not affected by EDTA | Enterobacter hormaechei | |
| 5.4.99.16 | Na+ | 90% residual activity at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | SDS | 10% residual activity at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | Zn2+ | 59% residual activity at 1 mM | Enterobacter hormaechei | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 25 | - |
maltose | in 50 mM phosphate buffer (pH 6) at 37°C | Enterobacter hormaechei | |
| 5.4.99.16 | 75 | - |
alpha,alpha-trehalose | in 50 mM phosphate buffer (pH 6) at 37°C | Enterobacter hormaechei | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | Mn2+ | Mn2+ shows an enhancing effect by 11% at 1 mM | Enterobacter hormaechei | |
| 5.4.99.16 | additional information | TreS activity is not affected by NH4+, Ca2+, and Mg2+ | Enterobacter hormaechei |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 61800 | - |
predicted from amino acid sequence | Enterobacter hormaechei |
| 5.4.99.16 | 65000 | - |
His-tagged enzyme, SDS-PAGE | Enterobacter hormaechei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Enterobacter hormaechei | B6E9W1 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | Ni-NTAcolumn chromatography | Enterobacter hormaechei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 18.5 | - |
purified enzyme, at 37°C | Enterobacter hormaechei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | - |
Enterobacter hormaechei | alpha,alpha-trehalose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | Trehalose synthase | - |
Enterobacter hormaechei |
| 5.4.99.16 | TreS | - |
Enterobacter hormaechei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 37 | - |
- |
Enterobacter hormaechei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 6 | - |
- |
Enterobacter hormaechei |
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Release 2023.1 (January 2023)
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