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Literature summary extracted from

  • Roy, H.; Ibba, M.
    Monitoring Lys-tRNA(Lys) phosphatidylglycerol transferase activity (2008), Methods, 44, 164-169.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.2.3 analysis a method to obtain a stable enriched membrane fraction containing MprF, and the techniques necessary to quantitatively monitor its activity in vitro and in vivo is reported Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.3 into the vector pET33b for expression in Escherichia coli Rosetta2 DE3, C41 and C43 cells Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.3 membrane
-
 Bacillus subtilis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.3 100000
-
 determined by SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol Bacillus subtilis
-
 tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.3 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.3 membrane fractions are prepared by differential centrifugation Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.3 0.00000072
-
 supernatant 10000 x g Bacillus subtilis
2.3.2.3 0.00000143
-
 cell crude extract Bacillus subtilis
2.3.2.3 0.00000326
-
 membrane fraction Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol
-
 Bacillus subtilis tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.3 Lys-tRNA(Lys) phosphatidylglycerol transferase
-
 Bacillus subtilis
2.3.2.3 MprF
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.3 37
-
 steady state assay Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.3 7.2
-
 steady state assay Bacillus subtilis