BRENDA - Enzyme Database

Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger

Meijer, S.; Otero, J.; Olivares, R.; Andersen, M.R.; Olsson, L.; Nielsen, J.; Metab. Eng. 11, 107-116 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
into vector pCR2.1-AfpyrG and overexpressed in the pyrA-strain AB4.1
Aspergillus niger
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Aspergillus niger
-
-
-
4.1.3.1
Aspergillus niger N402
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
705514
Aspergillus niger
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
705514
Aspergillus niger N402
succinate + glyoxylate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
into vector pCR2.1-AfpyrG and overexpressed in the pyrA-strain AB4.1
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
705514
Aspergillus niger
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
705514
Aspergillus niger N402
succinate + glyoxylate
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
ICL overexpression and malonate addition has a significant impact on metabolism and on organic acid production profiles. Increased ICL expression does not result in an increased glyoxylate bypass flux, there is a global response with respect to gene expression, leading to an increased flux through the oxidative part of the TCA cycle. Instead of an increased production of succinate and malate, a major increase in fumarate production is observed. In the strain with overexpression of ICL the organic acid production shifts from fumarate towards malate production when malonate is added to the cultivation medium
Aspergillus niger
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
ICL overexpression and malonate addition has a significant impact on metabolism and on organic acid production profiles. Increased ICL expression does not result in an increased glyoxylate bypass flux, there is a global response with respect to gene expression, leading to an increased flux through the oxidative part of the TCA cycle. Instead of an increased production of succinate and malate, a major increase in fumarate production is observed. In the strain with overexpression of ICL the organic acid production shifts from fumarate towards malate production when malonate is added to the cultivation medium
Aspergillus niger