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Literature summary extracted from

  • Coutinho, L.; Ferreira, M.A.; Cosson, A.; Batista, M.M.; Batista, D.d.a..G.; Minoprio, P.; Degrave, W.M.; Berneman, A.; Soeiro Mde, N.
    Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection (2009), Mem. Inst. Oswaldo Cruz, 104, 1055-1062.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.4 pyrrole-2-carboxylic acid inhibitor significantly affects parasite infection of Vero cells in vitro, inhibitor also hampers Trypanosoma cruzi intracellular differentiation, inhibitor reduces host cell invasion in Vero cells by Trypanososma cruzi in a dose-dependent manner, pre-treatment of the parasites with 1 mM of inhibitor does not lead to changes in their morphology and motility, but results in an up to 54% reduction in the percentage of parasitized cells and about 30% less parasites per cell when cultures are counted at day 4 after infection Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.4 cytoplasm TcPRAC localizes in the cytoplasm of intracellular amastigote forms of the parasite, near the flagellar pocket of the parasites Trypanosoma cruzi 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.4 L-proline Trypanosoma cruzi
-
 D-proline
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.4 Trypanosoma cruzi
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.1.4 epimastigote
-
 Trypanosoma cruzi
-
5.1.1.4 metacyclic form
-
 Trypanosoma cruzi
-
5.1.1.4 trypomastigote clone F11-F5 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.4 L-proline
-
 Trypanosoma cruzi D-proline
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.4 proline racemase
-
 Trypanosoma cruzi
5.1.1.4 TcPRAC
-
 Trypanosoma cruzi

Expression

EC Number Organism Comment Expression
5.1.1.4 Trypanosoma cruzi recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro additional information
5.1.1.4 Trypanosoma cruzi in amastigote forms of the parasites, the intensity of anti-TcPRAC labeling varies according to the time post infection reaching the highest signal of TcPRAC expression after 48 h, while the number of intracellular parasites increases by amastigote multiplication up

General Information

EC Number General Information Comment Organism
5.1.1.4 physiological function proline racemase is an effective mitogen for B cells, thus contributing to the parasites immune evasion and persistence in the human host Trypanosoma cruzi