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Literature summary extracted from

  • Balabanova, L.A.; Bakunina, I.Y.; Nedashkovskaya, O.I.; Makarenkova, I.D.; Zaporozhets, T.S.; Besednova, N.N.; Zvyagintseva, T.N.; Rasskazov, V.A.
    Molecular characterization and therapeutic potential of a marine bacterium Pseudoalteromonas sp. KMM 701 alpha-galactosidase (2010), Mar. Biotechnol., 12, 111-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Escherichia coli TOP10 or XL1 cells Pseudoalteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80055
-
 2 * 80055, calculated from amino acid sequence Pseudoalteromonas sp.
3.2.1.22 80300
-
 2 * 80300, SDS-PAGE Pseudoalteromonas sp.
3.2.1.22 170000
-
 gel filtration Pseudoalteromonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.22 melibiose + H2O Pseudoalteromonas sp.
-
 D-galactose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Pseudoalteromonas sp. Q19AX0 VKM B-2135 D
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sepharose CL-4B column chromatography, and Source 15Q PE gel filtration Pseudoalteromonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 0.11
-
 crude extract, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C Pseudoalteromonas sp.
3.2.1.22 231
-
 after 2100fold purification, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O highest activity Pseudoalteromonas sp. 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 4-nitrophenyl beta-D-galactopyranoside + H2O low activity Pseudoalteromonas sp. 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.22 melibiose + H2O
-
 Pseudoalteromonas sp. D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 homodimer 2 * 80055, calculated from amino acid sequence Pseudoalteromonas sp.
3.2.1.22 homodimer 2 * 80300, SDS-PAGE Pseudoalteromonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-D-galactosidase
-
 Pseudoalteromonas sp.
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 20 22
-
 Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 6.7 7.7
-
 Pseudoalteromonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Pseudoalteromonas sp. calculated from amino acid sequence
-
 5.6