BRENDA - Enzyme Database show

Diversity of the enzymatic activity in the lipoxygenase gene family of Arabidopsis thaliana

Bannenberg, G.; Martínez, M.; Hamberg, M.; Castresana, C.; Lipids 44, 85-95 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.12
expression in insect cells; expression in insect cells; expression in insect cells; expression in insect cells; recombinant baculovirus expressing LOX2 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells; recombinant baculovirus expressing LOX3 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells; recombinant baculovirus expressing LOX4 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells; recombinant baculovirus expressing LOX6 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
Arabidopsis thaliana
1.13.11.58
expression in insect cells; expression in insect cells
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Arabidopsis thaliana
P38418
-
-
1.13.11.12
Arabidopsis thaliana
Q9CAG3
-
-
1.13.11.12
Arabidopsis thaliana
Q9FNX8
-
-
1.13.11.12
Arabidopsis thaliana
Q9LNR3
-
-
1.13.11.58
Arabidopsis thaliana
Q06327
-
-
1.13.11.58
Arabidopsis thaliana
Q9LUW0
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
linoleate + O2
the enzyme oxygenates linolenic acid more effectively than linoleic acid
705472
Arabidopsis thaliana
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the enzyme forms exclusively (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
1.13.11.12
linolenate + O2
the enzyme oxygenates linolenic acid more effectively than linoleic acid
705472
Arabidopsis thaliana
?
the product is not determined
-
-
?
1.13.11.12
linolenic acid + O2
LOX-2 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-3 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-4 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-6 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
additional information
arachidonic acid is a poor substrate
705472
Arabidopsis thaliana
?
-
-
-
-
1.13.11.58
alpha-linolenate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
the enzyme forms exclusively (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
?
1.13.11.58
alpha-linolenate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
?
no product determined
-
-
?
1.13.11.58
linoleate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
the enzyme forms exclusively (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
?
1.13.11.58
additional information
arachidonic acid is a poor substrate
705472
Arabidopsis thaliana
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
expression in insect cells; recombinant baculovirus expressing LOX2 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
Arabidopsis thaliana
1.13.11.12
expression in insect cells; recombinant baculovirus expressing LOX3 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
Arabidopsis thaliana
1.13.11.12
expression in insect cells; recombinant baculovirus expressing LOX4 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
Arabidopsis thaliana
1.13.11.12
expression in insect cells; recombinant baculovirus expressing LOX6 (pFastBac) plasmid transferred into Escherichia coli DH10Bac containing the baculovirus shuttle vector bMON14272 and the helper plasmid pMON7124. Recombinant bacmid DNA transfected into High Five insect cells
Arabidopsis thaliana
1.13.11.58
expression in insect cells
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
linoleate + O2
the enzyme oxygenates linolenic acid more effectively than linoleic acid
705472
Arabidopsis thaliana
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the enzyme forms exclusively (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
1.13.11.12
linolenate + O2
the enzyme oxygenates linolenic acid more effectively than linoleic acid
705472
Arabidopsis thaliana
?
the product is not determined
-
-
?
1.13.11.12
linolenic acid + O2
LOX-2 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-3 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-4 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
linolenic acid + O2
LOX-6 displays a selective oxygenation of linolenic acid
705472
Arabidopsis thaliana
?
-
-
-
?
1.13.11.12
additional information
arachidonic acid is a poor substrate
705472
Arabidopsis thaliana
?
-
-
-
-
1.13.11.58
alpha-linolenate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
the enzyme forms exclusively (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
?
1.13.11.58
alpha-linolenate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
?
no product determined
-
-
?
1.13.11.58
linoleate + O2
comparable oxygenase activity with either linoleic acid or linolenic acid
705472
Arabidopsis thaliana
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
the enzyme forms exclusively (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
?
1.13.11.58
additional information
arachidonic acid is a poor substrate
705472
Arabidopsis thaliana
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
1.13.11.12
physiological function
LOX-2 is a 13S-lipoxygenases, it has no dual positional specificity; LOX-3 is a 13S-lipoxygenases, it has no dual positional specificity; LOX-4 is a 13S-lipoxygenases, it has no dual positional specificity; LOX-6 is a 13S-lipoxygenases, it has no dual positional specificity
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.12
physiological function
LOX-2 is a 13S-lipoxygenases, it has no dual positional specificity
Arabidopsis thaliana
1.13.11.12
physiological function
LOX-3 is a 13S-lipoxygenases, it has no dual positional specificity
Arabidopsis thaliana
1.13.11.12
physiological function
LOX-4 is a 13S-lipoxygenases, it has no dual positional specificity
Arabidopsis thaliana
1.13.11.12
physiological function
LOX-6 is a 13S-lipoxygenases, it has no dual positional specificity
Arabidopsis thaliana