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Literature summary extracted from

  • Pellon-Maison, M.; Garcia, C.F.; Cattaneo, E.R.; Coleman, R.A.; Gonzalez-Baro, M.R.
    Macrobrachium borellii hepatopancreas contains a mitochondrial glycerol-3-phosphate acyltransferase which initiates triacylglycerol biosynthesis (2009), Lipids, 44, 337-344.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 Polymyxin B
-
 Macrobrachium borellii

General Stability

EC Number General Stability Organism
2.3.1.15 since enzyme is unstable after long freezing periods, all experiments are performed within two weeks of fractionation Macrobrachium borellii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 palmitoleoyl-CoA shows substrate inhibition, therfore Km value is not determined Macrobrachium borellii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.0312
-
 oleoyl-CoA Vmax value calculated with oleoyl-CoA as a substrate is 4fold lower than the value obtained with palmitoyl-CoA Macrobrachium borellii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 mitochondrion higly active in mitochondria Macrobrachium borellii 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 70000
-
 SDS-PAGE Macrobrachium borellii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Macrobrachium borellii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 enzyme is isolated using subcellular fractions Macrobrachium borellii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 hepatopancreas in hepatopancreas mitochondria, a GPAT activity with characteristics similar to those of mammalian GPAT1 is identified Macrobrachium borellii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.000023
-
 specific activity in total homogenate Macrobrachium borellii
2.3.1.15 0.0000342
-
 specific activity in microsomes Macrobrachium borellii
2.3.1.15 0.000322
-
 specific activity in mitochondria Macrobrachium borellii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Macrobrachium borellii CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate preferred substrate is palmitoyl-CoA Macrobrachium borellii CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Macrobrachium borellii
2.3.1.15 GPAT
-
 Macrobrachium borellii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 30
-
 assay at Macrobrachium borellii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.4
-
 assay at Macrobrachium borellii