Any feedback?
Literature summary extracted from
- Expression and characterization of CYP51, the ancient sterol 14-demethylase activity for cytochromes P450 (CYP), in the white-rot fungus Phanerochaete chrysosporium (2008), Lipids, 43, 1143-1153.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.154 | cloned from a cDNA library into pGEMT-Easy vector. The pCWori+ vector transformed into Escherichia coli strain DH5alpha. Expressed in yeast YUG73 from pYES2.1 vector | Phanerodontia chrysosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.154 | (2R,4S)-ketoconazole | - |
Phanerodontia chrysosporium |  |
| 1.14.14.154 | clotrimazole | - |
Phanerodontia chrysosporium | |
| 1.14.14.154 | fluconazole | - |
Phanerodontia chrysosporium | |
| 1.14.14.154 | itraconazole | - |
Phanerodontia chrysosporium | |
| 1.14.14.154 | voriconazole | - |
Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.154 | 62000 | - |
sequence analysis | Phanerodontia chrysosporium |
| 1.14.14.154 | 63000 | - |
x * 63000, SDS-PAGE | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.154 | Phanerodontia chrysosporium | Q68HC3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.154 | by Ni2+-NTA affinity chromatography, more than 90% pure | Phanerodontia chrysosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.154 | -80°C | Phanerodontia chrysosporium |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | ? | x * 63000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | CYP51 | - |
Phanerodontia chrysosporium |
| 1.14.14.154 | sterol 14alpha-demethylase | - |
Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.154 | cytochrome P450 | - |
Phanerodontia chrysosporium |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.14.154 | Phanerodontia chrysosporium | sequence analysis | - |
6.27 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | physiological function | complementation of a conditional knock-down mutant of yeast CYP51. The CYP51 protein exhibits high affinity for azole antifungal compounds | Phanerodontia chrysosporium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
