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Literature summary extracted from

  • Sokolowsky, K.; Newton, M.; Lucero, C.; Wertheim, B.; Freedman, J.; Cortazar, F.; Czochor, J.; Schelvis, J.P.; Gindt, Y.M.
    Spectroscopic and Thermodynamic Comparisons of Escherichia coli DNA Photolyase and Vibrio cholerae Cryptochrome 1 (2010), J. Phys. Chem. B, 114, 7121-7130.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.99.3 FAD FAD binding pocket is similar to that of cryptochrome 1 Escherichia coli
4.1.99.3 FAD FAD binding pocket is similar to that of photolyase Vibrio cholerae serotype O1
4.1.99.3 FADH2 FADH-/FADH reduction potential of the cryptochrome 1 is significantly higher than that of the photolyase at 164 mV vs normal hydrogen electrode, but it also increases upon substrate binding (to 195 mV vs normal hydrogen electrode) similar to what is observed in photolyase. FADH-/FADH reduction potential is insensitive to ATP binding Vibrio cholerae serotype O1
4.1.99.3 FADH2 FADH-/FADH reduction potential of the cryptochrome1 is significantly higher than that of the photolyase at 164 mV vs normal hydrogen electrode, but it also increases upon substrate binding (to 195 mV vs normal hydrogen electrode) similar to what is observed in photolyase. FADH-/FADH reduction potential is insensitive to ATP binding Escherichia coli
4.1.99.3 additional information reduction potential is unchanged in the presence of 120× molar excess of Mg-ATP Escherichia coli
4.1.99.3 additional information reduction potential is unchanged in the presence of 120× molar excess of Mg-ATP Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.3 expressed from Escherichia coli strain UNC523F transfected with the pUNC2002 plasmid Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.3 Escherichia coli
-
-
-
4.1.99.3 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.3 isolated with a maltose binding domain, purified by ammonium sulfate precipitation and gel filtration Vibrio cholerae serotype O1

Storage Stability

EC Number Storage Stability Organism
4.1.99.3 -80°C Vibrio cholerae serotype O1
4.1.99.3 -80°C, for years Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.3 cyclobutadipyrimidine in DNA photolyase binds tighter to substrate than cryptochrome 1, binding constant is slightly sensitive to oxidation state Escherichia coli ?
-
 ?
4.1.99.3 cyclobutadipyrimidine in DNA photolyase binds tighter to substrate than cryptochrome 1, binding constant is slightly sensitive to oxidation state Vibrio cholerae serotype O1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.3 Cry-DASH
-
 Vibrio cholerae serotype O1
4.1.99.3 Cry1
-
 Vibrio cholerae serotype O1
4.1.99.3 cryptochrome 1
-
 Vibrio cholerae serotype O1
4.1.99.3 DNA photolyase
-
 Escherichia coli
4.1.99.3 photolyase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.3 0.073
-
 cyclobutadipyrimidine in DNA for dsDNA Escherichia coli
4.1.99.3 0.13
-
 cyclobutadipyrimidine in DNA for dsDNA Vibrio cholerae serotype O1
4.1.99.3 0.2167
-
 cyclobutadipyrimidine in DNA for ssDNA Escherichia coli
4.1.99.3 0.23
-
 cyclobutadipyrimidine in DNA for ssDNA Vibrio cholerae serotype O1