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Literature summary extracted from

  • Hamza, A.; Tong, M.; AbdulHameed, M.D.; Liu, J.; Goren, A.C.; Tai, H.H.; Zhan, C.G.
    Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis (2010), J. Phys. Chem. B, 114, 5605-5616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expressed in 293-F cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.3 H72A the mutation markedly decreases the mPGES-1 activity (32%) Homo sapiens
5.3.99.3 N74A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
5.3.99.3 R122A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
5.3.99.3 R70A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
5.3.99.3 R70A/R122A the mutant shows increased Km compared to the wild type enzyme, the double mutant of the mPGES-1 significantly reduces the binding affinity of GSH Homo sapiens
5.3.99.3 T129V the mutant shows increased Km compared to the wild type enzyme Homo sapiens
5.3.99.3 Y117A the mutant shows increased Km compared to the wild type enzyme Homo sapiens
5.3.99.3 Y117F the mutant still maintains full catalytic activity relative to the wild type Homo sapiens
5.3.99.3 Y117S the mutant shows increased Km compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.3 0.215
-
 prostaglandin H2 wild type enzyme Homo sapiens
5.3.99.3 0.2832
-
 prostaglandin H2 mutant enzyme Y117S Homo sapiens
5.3.99.3 0.296
-
 prostaglandin H2 mutant enzyme R70A Homo sapiens
5.3.99.3 0.372
-
 prostaglandin H2 mutant enzyme R122A Homo sapiens
5.3.99.3 0.3842
-
 prostaglandin H2 mutant enzyme T129V Homo sapiens
5.3.99.3 0.4643
-
 prostaglandin H2 mutant enzyme N74A Homo sapiens
5.3.99.3 0.4737
-
 prostaglandin H2 mutant enzyme R70A/R122A Homo sapiens
5.3.99.3 0.5345
-
 prostaglandin H2 mutant enzyme Y117A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 microsome
-
 Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Homo sapiens O14684
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin H2
-
 Homo sapiens Prostaglandin E2
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.3 trimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 microsomal prostaglandin E synthase-1
-
 Homo sapiens
5.3.99.3 mPGES-1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.3 glutathione
-
 Homo sapiens