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Literature summary extracted from

  • Pieper, D.; Seeger, M.
    Bacterial metabolism of polychlorinated biphenyls (2008), J. Mol. Microbiol. Biotechnol., 15, 121-138.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Paraburkholderia xenovorans LB400
-
-
-
1.14.12.18 Pseudomonas putida
-
-
-
1.14.12.18 Pseudomonas putida KF715
-
-
-
1.14.12.18 Rhodococcus sp.
-
-
-
1.14.12.18 Rhodococcus sp. RHA1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2
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 Paraburkholderia xenovorans LB400 2,3-dihydro-dihydroxybiphenyl + NAD+
-
 ?
1.14.12.18 additional information biphenyl pathway of strain LB400 oxidizes an unusually wide range of polychlorinated biphenyl congeners and BphA is able to dihydroxylate from mono-chlorobiphenyls to 2,3,4,5,2',5'-hexachlorobiphenyl, is able to oxidize other substituted biphenyls, unsubstituted dibenzofuran and dibenzodioxin and is also able to transform different isoflavonoids. A hybrid BphA based on the LB400 enzyme but harboring the core segment of an oxygenase from Pseudomonas sp. strain B4-Magdeburg shows (depending on the congener considered) complementing or improved degradative properties. BphA mutants with increased catalytic turnover on polychlorinated biphenyls, that are recalcitrant for the parental BphA from strain LB400 Paraburkholderia xenovorans LB400 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 Biphenyl 2,3-dioxygenase
-
 Pseudomonas putida
1.14.12.18 Biphenyl 2,3-dioxygenase
-
 Rhodococcus sp.
1.14.12.18 Biphenyl 2,3-dioxygenase
-
 Paraburkholderia xenovorans LB400
1.14.12.18 BphA
-
 Rhodococcus sp.
1.14.12.18 BphA
-
 Paraburkholderia xenovorans LB400
1.14.12.18 BphABCD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Paraburkholderia xenovorans LB400

Expression

EC Number Organism Comment Expression
1.14.12.18 Rhodococcus sp. bph gene cluster bphAaAbAcAdCB is localized on the linear plasmid pRHL1, which is followed by bphS and bphT genes which encode a two-component signal transduction system composed of a BphT response regulator and a BpdS sensor kinase and promote transcriptional induction by aromatic compounds biphenyl, benzene, alkylbenzene and chlorinated benzene up