EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.1 | expressed in Escherichia coli BL21(DE3) cells | Acetobacter pasteurianus |
4.1.1.1 | expressed in Escherichia coli BL21(DE3) cells | Zymobacter palmae |
EC Number | General Stability | Organism |
---|---|---|
4.1.1.1 | 0.1 mM thiamine diphosphate is sufficient to keep the enzyme stable and active in potassium phosphate buffer for several days | Saccharomyces cerevisiae |
4.1.1.1 | 0.1 mM thiamine diphosphate is sufficient to keep the enzyme stable and active in potassium phosphate buffer for several days | Acetobacter pasteurianus |
4.1.1.1 | 0.1 mM thiamine diphosphate is sufficient to keep the enzyme stable and active in potassium phosphate buffer for several days | Zymobacter palmae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.1 | 2.5 | - |
pyruvate | in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae | |
4.1.1.1 | 2.8 | - |
pyruvate | in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.1 | pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | Acetobacter pasteurianus | - |
acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | Zymobacter palmae | - |
acetaldehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.1 | Acetobacter pasteurianus | - |
- |
- |
4.1.1.1 | Saccharomyces cerevisiae | - |
- |
- |
4.1.1.1 | Zymobacter palmae | - |
strain DSMZ 10491 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 0.2 | - |
using 2-oxo-5-phenylpentanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 0.2 | - |
using 2-oxo-5-phenylpentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.3 | - |
using 2-oxo-4-phenylbutanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 0.3 | - |
using 2-oxo-4-phenylbutanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.3 | - |
using 3-fluoro-2-oxopropanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 0.3 | - |
using 4-methyl-2-oxopentanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 0.3 | - |
using oxo(phenyl)acetic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.4 | - |
using 4-methyl-2-oxohexanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.5 | - |
using 3-(1H-indol-3-yl)-2-oxopropanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.6 | - |
using 2-oxooctanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.6 | - |
using 3-fluoro-2-oxopropanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 0.6 | - |
using 4-methyl-2-oxohexanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 0.8 | - |
using phenylpyruvate as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 1.1 | - |
using 11 as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 1.1 | - |
using 2-oxooctanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 1.1 | - |
using 4-methyl-2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 1.7 | - |
using 2-oxo-4-phenylbutanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 1.8 | - |
using phenylpyruvate as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 2.2 | - |
using 3-methyl-2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 2.8 | - |
using 3-methyl-2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 2.8 | - |
using 4-methyl-2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 4.2 | - |
using 2-oxohexanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 5.3 | - |
using 2-oxohexanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 6.2 | - |
using 2-oxohexanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 6.9 | - |
using 3-methyl-2-oxobutanoate as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 10.4 | - |
using 3-methyl-2-oxobutanoate as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 12.9 | - |
using 2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 16.9 | - |
using 2-oxobutanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 18.8 | - |
using 2-oxopentanoic acid as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 20.7 | - |
using 2-oxopentanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 43.4 | - |
using pyruvate as substrate, in 100 mM potassium phosphate buffer pH 6.5, 5 mM MgSO4, 0.1 mM thiamine diphosphate | Saccharomyces cerevisiae |
4.1.1.1 | 60 | - |
using 2-oxobutanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 85.1 | - |
using 2-oxobutanoic acid as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
4.1.1.1 | 89.3 | - |
using pyruvate as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Acetobacter pasteurianus |
4.1.1.1 | 147 | - |
using pyruvate as substrate, in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate | Zymobacter palmae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.1 | 2-oxo-4-phenylbutanoic acid | - |
Saccharomyces cerevisiae | 3-phenylpropanal + CO2 | - |
? | |
4.1.1.1 | 2-oxo-4-phenylbutanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxo-4-phenylbutanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 2-oxo-5-phenylpentanoic acid | - |
Saccharomyces cerevisiae | 4-phenylbutanal + CO2 | - |
? | |
4.1.1.1 | 2-oxo-5-phenylpentanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxo-5-phenylpentanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 2-oxobutanoic acid | - |
Saccharomyces cerevisiae | propanal + CO2 | - |
? | |
4.1.1.1 | 2-oxobutanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxobutanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 2-oxohexanoic acid | - |
Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.1 | 2-oxohexanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxohexanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 2-oxohexanoic acid | - |
Saccharomyces cerevisiae | n-pentanal + CO2 | - |
? | |
4.1.1.1 | 2-oxooctanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxooctanoic acid | - |
Zymobacter palmae | n-hexanal + CO2 | - |
? | |
4.1.1.1 | 2-oxopentanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 2-oxopentanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 2-oxopentanoic acid | - |
Saccharomyces cerevisiae | n-butanal + CO2 | - |
? | |
4.1.1.1 | 3-(1H-indol-3-yl)-2-oxopropanoic acid | - |
Zymobacter palmae | 1H-indol-3-ylacetaldehyde + CO2 | - |
? | |
4.1.1.1 | 3-(1H-indol-3-yl)-2-oxopropanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 3-fluoro-2-oxopropanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 3-fluoro-2-oxopropanoic acid | - |
Zymobacter palmae | fluoroacetaldehyde + CO2 | - |
? | |
4.1.1.1 | 3-methyl-2-oxobutanoate | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 3-methyl-2-oxopentanoic acid | - |
Zymobacter palmae | 2-methylbutanal + CO2 | - |
? | |
4.1.1.1 | 3-methyl-2-oxopentanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 3-methyl-2-oxopentanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 4-methyl-2-oxohexanoic acid | - |
Zymobacter palmae | 3-methylpentanal + CO2 | - |
? | |
4.1.1.1 | 4-methyl-2-oxohexanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | 4-methyl-2-oxohexanoic acid | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | 4-methyl-2-oxopentanoic acid | - |
Saccharomyces cerevisiae | 3-methylbutanal + CO2 | - |
? | |
4.1.1.1 | 4-methyl-2-oxopentanoic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | acetaldehyde + acetaldehyde | - |
Saccharomyces cerevisiae | (S)-acetoin + ? | - |
? | |
4.1.1.1 | acetaldehyde + acetaldehyde | - |
Acetobacter pasteurianus | (S)-acetoin + ? | - |
? | |
4.1.1.1 | acetaldehyde + acetaldehyde | - |
Zymobacter palmae | (S)-acetoin + ? | - |
? | |
4.1.1.1 | acetaldehyde + benzaldehyde | - |
Saccharomyces cerevisiae | (R)-phenylacetylcarbinol | - |
? | |
4.1.1.1 | acetaldehyde + benzaldehyde | - |
Acetobacter pasteurianus | (R)-phenylacetylcarbinol | - |
? | |
4.1.1.1 | acetaldehyde + benzaldehyde | - |
Zymobacter palmae | (R)-phenylacetylcarbinol | - |
? | |
4.1.1.1 | additional information | does not act on phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.1 | additional information | even with benzaldehyde as the only substrate no benzoin can be detected, the enzyme does not produce (S)-2-hydroxypropiophenone | Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | additional information | even with benzaldehyde as the only substrate no benzoin can be detected, the enzyme does not produce (S)-2-hydroxypropiophenone | Zymobacter palmae | ? | - |
? | |
4.1.1.1 | oxo(phenyl)acetic acid | - |
Zymobacter palmae | benzaldehyde + CO2 | - |
? | |
4.1.1.1 | oxo(phenyl)acetic acid | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | Phenylpyruvate | - |
Acetobacter pasteurianus | ? | - |
? | |
4.1.1.1 | Phenylpyruvate | - |
Zymobacter palmae | ? | - |
? | |
4.1.1.1 | pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | - |
Acetobacter pasteurianus | acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | - |
Zymobacter palmae | acetaldehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.1 | PDC | - |
Saccharomyces cerevisiae |
4.1.1.1 | PDC | - |
Acetobacter pasteurianus |
4.1.1.1 | PDC | - |
Zymobacter palmae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 43 | - |
- |
Saccharomyces cerevisiae |
4.1.1.1 | 55 | - |
- |
Zymobacter palmae |
4.1.1.1 | 65 | - |
- |
Acetobacter pasteurianus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 20 | 35 | half-life of 235 h at 20°C, half-life of 78 h at 30°C, half-life of 62 h at 35°C | Saccharomyces cerevisiae |
4.1.1.1 | 30 | 70 | half-life of 144 h at 30°C, 34 h at 40°C, 12 h at 50°C, 2 h at 60°C, and 0.4 h at 70°C | Acetobacter pasteurianus |
4.1.1.1 | 30 | 60 | half-life of 150 h at 30°C, 40 h at 40°C, 10 h at 50°C, and 0.4 h at 60°C | Zymobacter palmae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 3.5 | 6.5 | - |
Acetobacter pasteurianus |
4.1.1.1 | 5 | 7 | - |
Saccharomyces cerevisiae |
4.1.1.1 | 7 | - |
- |
Zymobacter palmae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 4.5 | 8 | - |
Zymobacter palmae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 4 | 9 | the enzyme is stable at pH 6.5/8.0 for several days, complete activity loss occurs within 2 h at pH 4.0 or 9.0 | Zymobacter palmae |
4.1.1.1 | 5 | 7 | the enzyme shows no activity loss at pH 5.8-7.0 within 60 h, half-life at pH 4.0 is 2.3 h | Acetobacter pasteurianus |
4.1.1.1 | 5 | 8 | half-life of 80 h at pH 5, half-life of 53 h at pH 7.0, half-life of 13 h at pH 8.0 | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.1 | thiamine diphosphate | dependent on | Saccharomyces cerevisiae | |
4.1.1.1 | thiamine diphosphate | dependent on | Acetobacter pasteurianus | |
4.1.1.1 | thiamine diphosphate | dependent on | Zymobacter palmae |