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Literature summary extracted from
- Properties of a novel beta-glucosidase from Fusarium proliferatum ECU2042 that converts ginsenoside Rg3 into Rh2 (2009), J. Mol. Catal. B, 57, 278-283.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ca2+ | 73% residual activity at 10 mM | Fusarium proliferatum |  |
| 3.2.1.21 | EDTA | 67% residual activity at 10 mM | Fusarium proliferatum | |
| 3.2.1.21 | Mg2+ | 75% residual activity at 10 mM | Fusarium proliferatum | |
| 3.2.1.21 | Mn2+ | 77% residual activity at 10 mM | Fusarium proliferatum | |
| 3.2.1.21 | Ni2+ | 74% residual activity at 10 mM | Fusarium proliferatum | |
| 3.2.1.21 | Zn2+ | 92% residual activity at 10 mM | Fusarium proliferatum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 2.37 | - |
ginsenoside Rg3 | in NaAc/HAc buffer (0.2 M, pH 5.0), at 50°C | Fusarium proliferatum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Co2+ | 114% relative activity at 10 mM | Fusarium proliferatum | |
| 3.2.1.21 | additional information | the enzyme is not apparently affected by heavy metal salts, such as Cu2+ and Fe3+ | Fusarium proliferatum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 39100 | - |
2 * 39100, SDS-PAGE | Fusarium proliferatum |
| 3.2.1.21 | 78700 | - |
gel filtration | Fusarium proliferatum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Fusarium proliferatum | - |
- |
- |
| 3.2.1.21 | Fusarium proliferatum ECU2042 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | ammonium sulfate precipitation, butyl-Toyopearl 650M column chromatography, DEAE-Toyopearl 650M column chromatography, TSK gel filtration | Fusarium proliferatum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 0.000081 | - |
crude extract, at pH 5.0 and 50°C | Fusarium proliferatum |
| 3.2.1.21 | 0.041 | - |
after 506fold purification, at pH 5.0 and 50°C | Fusarium proliferatum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-chlorophenyl beta-D-glucopyranoside + H2O | 227% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | 4-chlorophenyl beta-D-glucopyranoside + H2O | 227% activity compared to ginsenoside Rg3 | Fusarium proliferatum ECU2042 | ? | - |
? | |
| 3.2.1.21 | 4-hydroxybenzyl beta-D-glucopyranoside + H2O | 551% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | 4-hydroxybenzyl beta-D-glucopyranoside + H2O | 551% activity compared to ginsenoside Rg3 | Fusarium proliferatum ECU2042 | ? | - |
? | |
| 3.2.1.21 | 4-methoxybenzyl beta-D-glucopyranoside + H2O | 73.2% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | 4-methoxybenzyl beta-D-glucopyranoside + H2O | 73.2% activity compared to ginsenoside Rg3 | Fusarium proliferatum ECU2042 | ? | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | very low activity | Fusarium proliferatum | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | very low activity | Fusarium proliferatum ECU2042 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | barbaloin + H2O | 63% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | butyl beta-D-glucopyranoside + H2O | 39% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | cyclopentyl beta-D-glucopyranoside + H2O | 65.9% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | esculin hydrate + H2O | 31.7% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | ethyl beta-D-glucopyranoside + H2O | 14.6% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | ginsenoside Rg3 + H2O | 100% activity, the enzyme hydrolyzes the 3-C, beta-(1-2)-glucoside of ginsenoside Rg3 to produce ginsenoside Rh2, but does not sequentially hydrolyze the beta-D-glucosidic bond of ginsenoside Rh2 | Fusarium proliferatum | ginsenoside Rh2 + D-glucose | - |
? | |
| 3.2.1.21 | hexyl beta-D-glucopyranoside + H2O | 53.7% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | isopropyl beta-D-glucopyranoside + H2O | 9.8% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | additional information | the purified enzyme exhibits almost no activity towards cellobiose and 4-nitrophenyl-beta-D-galactopyranoside, the enzyme does not hydrolyze 4-bromophenyl beta-D-glucopyranoside and ginsenoside Rh2 after incubation at 50°C for 24 h | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | additional information | the purified enzyme exhibits almost no activity towards cellobiose and 4-nitrophenyl-beta-D-galactopyranoside, the enzyme does not hydrolyze 4-bromophenyl beta-D-glucopyranoside and ginsenoside Rh2 after incubation at 50°C for 24 h | Fusarium proliferatum ECU2042 | ? | - |
? | |
| 3.2.1.21 | propenyl beta-D-glucopyranoside + H2O | 26.8% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | propyl beta-D-glucopyranoside + H2O | 31.7% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
| 3.2.1.21 | salidroside + H2O | 51.2% activity compared to ginsenoside Rg3 | Fusarium proliferatum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | homodimer | 2 * 39100, SDS-PAGE | Fusarium proliferatum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | FPG | - |
Fusarium proliferatum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Fusarium proliferatum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 60 | - |
Fusarium proliferatum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60 | - |
stable at temperatures below 60°C. The half-lives of the purified enzyme at 30, 40, 50, 60 and 70°C are 141.4, 90.0, 58.7, 27.1 and 1.4 h, respectively | Fusarium proliferatum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Fusarium proliferatum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 6 | - |
Fusarium proliferatum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 6.5 | the enzyme activity remains more than 70% after incubating at pH 4.0-6.5 at 4°C for 24 h | Fusarium proliferatum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Fusarium proliferatum | isoelectric focusing | - |
4.4 |
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