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Literature summary extracted from

  • Merritt, E.A.; Arakaki, T.L.; Gillespie, J.R.; Larson, E.T.; Kelley, A.; Mueller, N.; Napuli, A.J.; Kim, J.; Zhang, L.; Verlinde, C.L.; Fan, E.; Zucker, F.; Buckner, F.S.; van Voorhis, W.C.; Hol, W.G.
    Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs (2010), J. Mol. Biol., 397, 481-494.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 the full-length and a truncated variant is cloned into the Escherichia coli expression vector AVA0421 derived from vector pET14b Trypanosoma brucei
6.1.1.21 the full-length and a truncated variant is cloned into the Escherichia coli expression vector AVA0421 derived from vector pET14b Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.21 the structure of histidyl-tRNA synthetase in complex with L-histidine is determined to a resolution of 1.8 A , in complex with histidyl-adenosinemonophosphate to a resolution of 3.05 A Trypanosoma cruzi
6.1.1.21 the structure of histidyl-tRNA synthetase is determined to a resolution of 2.7 A Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 RNAi
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Trypanosoma brucei
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis Trypanosoma cruzi
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Trypanosoma brucei Q4DA54 residues 44-477
-
6.1.1.21 Trypanosoma cruzi Q4DA54 residues 45-478
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21 by a Ni-NTA affinity column followed by a XK26/60 Superdex 75 column Trypanosoma brucei
6.1.1.21 by a Ni-NTA affinity column followed by a XK26/60 Superdex 75 column Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Trypanosoma brucei AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Trypanosoma cruzi AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Trypanosoma brucei
6.1.1.21 HisRS
-
 Trypanosoma cruzi
6.1.1.21 Histidyl-tRNA synthetase
-
 Trypanosoma brucei
6.1.1.21 Histidyl-tRNA synthetase
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Trypanosoma brucei
6.1.1.21 ATP
-
 Trypanosoma cruzi