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Literature summary extracted from

  • Larson, E.T.; Mudeppa, D.G.; Gillespie, J.R.; Mueller, N.; Napuli, A.J.; Arif, J.A.; Ross, J.; Arakaki, T.L.; Lauricella, A.; Detitta, G.; Luft, J.; Zucker, F.; Verlinde, C.L.; Fan, E.; Van Voorhis, W.C.; Buckner, F.S.; Rathod, P.K.; Hol, W.G.; Merritt, E.A.
    The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase (2010), J. Mol. Biol., 396, 1244-1259.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 free selenomethionine-labeled enzyme, or enzyme in complex with uridine, X-ray diffraction structure determination and analysis at 2.7 A and 1.44 A resolution, respectively Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.3 additional information 90% suppression of enzyme expression by RNAi does not lead to growth inhibition Trypanosoma brucei

General Stability

EC Number General Stability Organism
2.4.2.3 Ca2+ or EGTA stabilize the enzyme Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.3 Ca2+ stabilizes the enzyme, which possesses an intermolecular metal-binding site, structure, overview Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Trypanosoma brucei
-
 uracil + alpha-D-ribose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Trypanosoma brucei Q57VZ2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 additional information
-
 pH dependence of enzyme activities with different substrates, overview Trypanosoma brucei
2.4.2.3 1.44
-
 substrate deoxyuridine, pH 6.5 Trypanosoma brucei
2.4.2.3 2.75
-
 substrate uridine, pH 7.5 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 deoxyuridine + phosphate 14% of the activity with uridine Trypanosoma brucei uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 guanosine + phosphate 10% of the activity with uridine Trypanosoma brucei guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 additional information strict substrate specificty of the enzyme, overview. Deoxyadenosine, and deoxyguanosine are poor substrates, thymidine, cytidine, deoxycytidine, inosine, and adenosine are no substrates Trypanosoma brucei ?
-
 ?
2.4.2.3 uridine + phosphate
-
 Trypanosoma brucei uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 uridine + phosphate best substrate, active site structure, overview Trypanosoma brucei uracil + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.3 homodimer each monomer is composed of a central 11-stranded mixed beta-sheet surrounded by 14 alpha-helices and two peripheral short, 2-stranded antiparallel beta-sheets Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 More the enzyme belongs to the NP1 subfamily of the nucleoside phosphorylase, NP, superfamily of enzymes Trypanosoma brucei
2.4.2.3 uridine phosphorylase
-
 Trypanosoma brucei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.3 55
-
 inactivation after 50 min in presence of 2 mM EGTA, 60% of maximal activity remaining after 90 min in presence of Ca2+ Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 6.5
-
 with substrate deoxyuridine Trypanosoma brucei
2.4.2.3 7.5
-
 with substrate uridine Trypanosoma brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.3 6 8.5 pH dependence of enzyme activities with different substrates, overview Trypanosoma brucei