BRENDA - Enzyme Database

Crystal structures of Leishmania mexicana phosphoglycerate mutase suggest a one-metal mechanism and a new enzyme subclass

Nowicki, M.W.; Kuaprasert, B.; McNae, I.W.; Morgan, H.P.; Harding, M.M.; Michels, P.A.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.; J. Mol. Biol. 394, 535-543 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
His-tagged enzyme is expressed in Escherichia coli BL21 cells
Leishmania mexicana
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
iPGAM crystallized with 3-phosphoglycerate or 2-phosphoglycerate at high and low cobalt concentrations (4 mM and 0.01 mM), hanging drop vapor diffusion method, using 0.08 M ammonium acetate, 0.04 M trisodium citrate dihydrate pH 6.0, and 24% (w/v) PEG 4000
Leishmania mexicana
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Co2+
two Co2+ are coordinated within the active site
Leishmania mexicana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Leishmania mexicana
Q86N96
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
metal affinity Talon column chromatography
Leishmania mexicana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
705169
Leishmania mexicana
3-phospho-D-glycerate
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
phosphoglycerate mutase is cofactor-independent
Leishmania mexicana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
His-tagged enzyme is expressed in Escherichia coli BL21 cells
Leishmania mexicana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
phosphoglycerate mutase is cofactor-independent
Leishmania mexicana
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
iPGAM crystallized with 3-phosphoglycerate or 2-phosphoglycerate at high and low cobalt concentrations (4 mM and 0.01 mM), hanging drop vapor diffusion method, using 0.08 M ammonium acetate, 0.04 M trisodium citrate dihydrate pH 6.0, and 24% (w/v) PEG 4000
Leishmania mexicana
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Co2+
two Co2+ are coordinated within the active site
Leishmania mexicana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
metal affinity Talon column chromatography
Leishmania mexicana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
705169
Leishmania mexicana
3-phospho-D-glycerate
-
-
-
r