EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.3.6 | into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells | Eubacterium barkeri |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.3.6 | the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A | Eubacterium barkeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.6 | 7 | - |
methylitaconate | (R,S)-3-methylitaconate | Eubacterium barkeri | |
5.3.3.6 | 60 | - |
Itaconate | - |
Eubacterium barkeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.3.6 | 40270 | - |
theoretical, without Strep-tag | Eubacterium barkeri |
5.3.3.6 | 41500 | - |
theoretical, including the Strep-tag | Eubacterium barkeri |
5.3.3.6 | 45000 | - |
apparent molecular mass, determined by SDS-PAGE | Eubacterium barkeri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.6 | methylitaconate | Eubacterium barkeri | - |
2,3-dimethylmaleate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.3.6 | Eubacterium barkeri | Q0QLE6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.3.6 | affinity purification on a streptavidin column | Eubacterium barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.6 | itaconate | - |
Eubacterium barkeri | methylmaleate | - |
? | |
5.3.3.6 | methylitaconate | - |
Eubacterium barkeri | 2,3-dimethylmaleate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.3.6 | homotetramer | 4 * 40270 Da | Eubacterium barkeri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.3.6 | 3-methylitaconate-DELTA-isomerase | - |
Eubacterium barkeri |
5.3.3.6 | MII | - |
Eubacterium barkeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.3.6 | 25 | - |
activity assay | Eubacterium barkeri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.6 | 2 | - |
Itaconate | - |
Eubacterium barkeri | |
5.3.3.6 | 1720 | - |
methylitaconate | (R,S)-3-methylitaconate | Eubacterium barkeri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.3.6 | 7.4 | - |
activity assay | Eubacterium barkeri |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.3.6 | metabolism | 3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway | Eubacterium barkeri |