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Literature summary extracted from

  • Velarde, M.; Macieira, S.; Hilberg, M.; Bröker, G.; Tu, S.-M.; Golding, B.T.; Pierik, A.J.; Buckel, W.; Messerschmidt, A.
    Crystal structure and putative mechanism of 3-methylitaconate-DELTA-isomerase from Eubacterium barkeri (2009), J. Mol. Biol., 391, 609-620.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.6 into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells Eubacterium barkeri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.6 the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A Eubacterium barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.6 7
-
methylitaconate (R,S)-3-methylitaconate Eubacterium barkeri
5.3.3.6 60
-
Itaconate
-
Eubacterium barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.6 40270
-
theoretical, without Strep-tag Eubacterium barkeri
5.3.3.6 41500
-
theoretical, including the Strep-tag Eubacterium barkeri
5.3.3.6 45000
-
apparent molecular mass, determined by SDS-PAGE Eubacterium barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.6 methylitaconate Eubacterium barkeri
-
2,3-dimethylmaleate
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.6 Eubacterium barkeri Q0QLE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.6 affinity purification on a streptavidin column Eubacterium barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.6 itaconate
-
Eubacterium barkeri methylmaleate
-
?
5.3.3.6 methylitaconate
-
Eubacterium barkeri 2,3-dimethylmaleate
-
r

Subunits

EC Number Subunits Comment Organism
5.3.3.6 homotetramer 4 * 40270 Da Eubacterium barkeri

Synonyms

EC Number Synonyms Comment Organism
5.3.3.6 3-methylitaconate-DELTA-isomerase
-
Eubacterium barkeri
5.3.3.6 MII
-
Eubacterium barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.6 25
-
activity assay Eubacterium barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.6 2
-
Itaconate
-
Eubacterium barkeri
5.3.3.6 1720
-
methylitaconate (R,S)-3-methylitaconate Eubacterium barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.6 7.4
-
activity assay Eubacterium barkeri

General Information

EC Number General Information Comment Organism
5.3.3.6 metabolism 3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway Eubacterium barkeri