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Literature summary extracted from

  • Osman, K.T.; Du, L.; He, Y.; Luo, Y.
    Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP (2009), J. Mol. Biol., 388, 345-355.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.54 the pET28a vector is used Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.54 the crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) is determined in complex with ATP to 1.9 A resolution Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.54 D383N mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies Bacillus cereus
6.2.1.54 E298Q mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies Bacillus cereus
6.2.1.54 R397Q mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.54 0.01
-
 ATP wild-type Bacillus cereus
6.2.1.54 0.06
-
 ATP mutant R397Q Bacillus cereus
6.2.1.54 0.08
-
 ATP mutant E298Q Bacillus cereus
6.2.1.54 0.75
-
 ATP mutant D383N Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.54 Mg2+
-
 Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.54 ATP + D-alanine + poly(ribitol phosphate) Bacillus cereus
-
 AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.54 Bacillus cereus Q81G39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.54
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.54 ATP + D-alanine + poly(ribitol phosphate)
-
 Bacillus cereus AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.54 D-alanyl carrier protein ligase
-
 Bacillus cereus
6.2.1.54 DltA
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.54 0.011
-
 ATP mutant R397Q Bacillus cereus
6.2.1.54 0.016
-
 ATP mutant E298Q Bacillus cereus
6.2.1.54 0.097
-
 ATP mutant D383N Bacillus cereus
6.2.1.54 0.215
-
 ATP wild-type Bacillus cereus