Literature summary extracted from

Xu, G.; Ryan, C.; Kiefel, M.J.; Wilson, J.C.; Taylor, G.L.
Structural studies on the Pseudomonas aeruginosa sialidase-like enzyme PA2794 suggest substrate and mechanistic variations (2009), J. Mol. Biol., 386, 828-840.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.18	expressed in Escherichia coli BL21(DE3) cells, the recombinant enzyme does not show any sialidase activity with the standard fluorogenic sialic-acid-based substrate	Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.18	hanging drop vapor diffusion method, using 20% PEG 6K and 0.1 M bicine, pH 5.0	Pseudomonas aeruginosa

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.18	Pseudomonas aeruginosa	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.18	2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O	-	Pseudomonas aeruginosa	4-methylumbelliferone + N-acetylneuraminic acid	-	?
3.2.1.18	alpha(2-3)-sialyllactose + H2O	-	Pseudomonas aeruginosa	sialic acid + lactose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.18	trimer	x-ray crystallography	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.18	PA2794	-	Pseudomonas aeruginosa
3.2.1.18	sialidase/neuraminidase mutant F129A	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)