Literature summary extracted from

Messens, J.; Van Molle, I.; Vanhaesebrouck, P.; Limbourg, M.; Van Belle, K.; Wahni, K.; Martins, J.C.; Loris, R.; Wyns, L.
How thioredoxin can reduce a buried disulphide bond (2004), J. Mol. Biol., 339, 527-537.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.20.4.4	C10S/C15A/C89L mutant and C10S/C15A/C82S mutant expressed in Escherichia coli	Staphylococcus aureus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.20.4.4	C10S/C15A/C82S	mutant, determination of the redox potential of the Cys82-Cys89 redox couple, thioredoxin is unable to reduce the Cys10-Cys15 disulfide in oxidized ArsC C82S	Staphylococcus aureus
1.20.4.4	C10S/C15A/C89L	mutant, determination of the redox potential of the Cys82-Cys89 redox couple	Staphylococcus aureus
1.20.4.4	additional information	ArsC triple mutants and the Trx C32S mutant formate a ArsC-TrxC32S complex	Staphylococcus aureus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.20.4.4	0.033	-	thioredoxin	-	Staphylococcus aureus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.20.4.4	14800	-	reduced C82S mutant	Staphylococcus aureus
1.20.4.4	14810	-	reducted wild-type	Staphylococcus aureus
1.20.4.4	14820	-	reduced C89L mutant	Staphylococcus aureus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.20.4.4	Staphylococcus aureus	P0A006	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.20.4.4	C10S/C15A/C89L mutant and C10S/C15A/C82S mutant purified by Ni2+-NTA Superflow	Staphylococcus aureus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.20.4.4	arsenate + thioredoxin	-	Staphylococcus aureus	arsenite + thioredoxin disulfide + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.20.4.4	ArsC	-	Staphylococcus aureus
1.20.4.4	pI258 arsenate reductase	-	Staphylococcus aureus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.20.4.4	1.9	-	thioredoxin	-	Staphylococcus aureus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.20.4.4	thioredoxin	interaction between thioredoxin and the enzyme pI258 arsenate reductase. Thioredoxin converts oxidized ArsC back towards its initial reduced state	Staphylococcus aureus

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.20.4.4	57.6	-	thioredoxin	-	Staphylococcus aureus

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Release 2023.1 (January 2023)