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Literature summary extracted from
- Cloning, expression, and characterization of thermostable manganese superoxide dismutase from Thermoascus aurantiacus var. levisporus (2009), J. Microbiol., 47, 123-130.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | gene sod, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparison, phylogenetic tree, inducible expression in Pichia pastoris strain GS115 using the AOX1 promoter | Thermoascus aurantiacus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | additional information | no inhibition by KCN and H2O2 | Thermoascus aurantiacus | |
| 1.15.1.1 | NaN3 | - |
Thermoascus aurantiacus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.15.1.1 | mitochondrion | the enzyme possesses a unique 35 amino acid mitochondrial targeting sequence at the N-terminus | Thermoascus aurantiacus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | Mn | a manganese superoxide dismutase, contains 0.00246 mg Mn/mg protein, binding involves conserved residues H88, H136, D222, and H226 | Thermoascus aurantiacus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 21700 | - |
4 * 21700, SDS-PAGE | Thermoascus aurantiacus |
| 1.15.1.1 | 68000 | - |
gel filtration | Thermoascus aurantiacus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | Thermoascus aurantiacus | A3RLW7 | var. levisporus, gene sod | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | recombinant enzyme from Pichia pastoris strain GS115 by anion exchange chromatography | Thermoascus aurantiacus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | mycelium | - |
Thermoascus aurantiacus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 2324 | - |
purified recombinant enzyme | Thermoascus aurantiacus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | tetramer | 4 * 21700, SDS-PAGE | Thermoascus aurantiacus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | manganese superoxide dismutase | - |
Thermoascus aurantiacus |
| 1.15.1.1 | MnSOD | - |
Thermoascus aurantiacus |
| 1.15.1.1 | SOD | - |
Thermoascus aurantiacus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 55 | - |
recombinant enzyme | Thermoascus aurantiacus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 50 | 90 | - |
Thermoascus aurantiacus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 50 | 60 | purified recombinant enzyme, stable | Thermoascus aurantiacus |
| 1.15.1.1 | 70 | - |
purified recombinant enzyme, 65% remaining activity after 60 min | Thermoascus aurantiacus |
| 1.15.1.1 | 80 | - |
purified recombinant enzyme, half-life is about 40 min | Thermoascus aurantiacus |
| 1.15.1.1 | 90 | - |
purified recombinant enzyme, 20% remaining activity after 50 min | Thermoascus aurantiacus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 7.5 | - |
recombinant enzyme | Thermoascus aurantiacus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 6.5 | 9 | activity range | Thermoascus aurantiacus |
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