Literature summary extracted from
Wu, S.; Shen, R.; Zhang, X.; Wang, Q.
Molecular cloning and characterization of maltooligosyltrehalose synthase gene from Nostoc flagelliforme (2010), J. Microbiol. Biotechnol., 20, 579-586.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.99.15 |
expressed in Escherichia coli BL21(DE3) cells |
Nostoc flagelliforme |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.4.99.15 |
Cu2+ |
54% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
5.4.99.15 |
EDTA |
31% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
5.4.99.15 |
Hg2+ |
42% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
5.4.99.15 |
Mg2+ |
66% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
5.4.99.15 |
Mn2+ |
51% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.99.15 |
1.87 |
- |
maltohexaose |
recombinant enzyme, in 50 mM phosphate-citric acid buffer (0.2 M Na3PO4, 0.1 M citric acid, pH 7.0) at 50°C |
Nostoc flagelliforme |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.4.99.15 |
Ca2+ |
134% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
5.4.99.15 |
Zn2+ |
122% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) |
Nostoc flagelliforme |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.4.99.15 |
106600 |
- |
calculated from amino acid sequence |
Nostoc flagelliforme |
5.4.99.15 |
125000 |
- |
His6-tagged enzyme, SDS-PAGE |
Nostoc flagelliforme |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.99.15 |
Nostoc flagelliforme |
EF433294 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.99.15 |
Ni2+-NTA-agarose column chromatography |
Nostoc flagelliforme |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.99.15 |
maltohexaose |
- |
Nostoc flagelliforme |
alpha-maltotetraosyl trehalose |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.99.15 |
maltooligosyltrehalose synthase |
- |
Nostoc flagelliforme |
5.4.99.15 |
MTS |
- |
Nostoc flagelliforme |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.4.99.15 |
50 |
- |
recombinant enzyme |
Nostoc flagelliforme |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.4.99.15 |
7 |
- |
recombinant enzyme |
Nostoc flagelliforme |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
5.4.99.15 |
6 |
8 |
- |
Nostoc flagelliforme |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
5.4.99.15 |
Nostoc flagelliforme |
the expression of MTS is upregulated during drought stress |
up |