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Literature summary extracted from

  • Jin, B.S.; Han, S.G.; Lee, W.K.; Ryoo, S.W.; Lee, S.J.; Suh, S.W.; Yu, Y.G.
    Inhibitory mechanism of novel inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae (2009), J. Microbiol. Biotechnol., 19, 1582-1589.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 coding region of Haemophilus influenzae MurA is cloned into the NdeI and XhoI sites of pET21a to generate an expression vector for MurA, the expression vector is transformed into Escherichia coli Rosetta2 (DE3), and the recombinant MurA is expressed Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 ebselen inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated Haemophilus influenzae
2.5.1.7 fosfomycin
-
 Haemophilus influenzae
2.5.1.7 thimerosal inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated Haemophilus influenzae
2.5.1.7 thiram inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Haemophilus influenzae P45025
-
-

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 MurA
-
 Haemophilus influenzae
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.7 25
-
 assay at Haemophilus influenzae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.7 0.0001
-
 50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM Haemophilus influenzae ebselen
2.5.1.7 0.0004
-
 50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM Haemophilus influenzae thimerosal
2.5.1.7 0.0007
-
 50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM Haemophilus influenzae thiram
2.5.1.7 0.04
-
 50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM Haemophilus influenzae fosfomycin

General Information

EC Number General Information Comment Organism
2.5.1.7 physiological function enzyme catalyzes the first step of bacterial cell wall synthesis Haemophilus influenzae